基于线粒体控制区Dloop序列的长臀种群遗传结构分析  

Analysis of population genetic structure of Cranoglanis based on mitochondrial Dloop sequences

作　　者：谢少林[1] 王超[1,2] 吕子君[1] 李正光[1] 石忍耐 邹记兴[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]清远市兴渔水产科技有限公司,广东清远511510

出　　处：《华南农业大学学报》2016年第1期8-13,共6页Journal of South China Agricultural University

基　　金：广东省海洋渔业科技推广专项科技攻关与研发项目(A201301F03);广东省海洋渔业科技与产业发展专项(A201501A02);科技型中小企业技术创新基金(14C26214402655)

摘　　要：【目的】了解长臀Cranoglanis 3个种群的野生资源状况,并对3个种群的物种有效性进行分析。【方法】采用线粒体控制区Dloop基因序列测定技术,分析了珠江水系、海南水系和越南红河水系长臀种群的群体遗传结构及其变异。【结果】在检测的84个个体中共得到43个单倍型,呈现出较高的单倍型多样性与较为贫乏的核苷酸多样性,其中海南长臀C.multiradiatus群体的单倍型多样性(Hd=0.871)和核苷酸多样性(Pi=0.006 4)最低;Tajima’s D中性检验以及核苷酸不配对分析均表明,3个长臀群体趋于稳定,未经历过大规模的种群扩张。Fst分析发现,海南长臀同珠江长臀C.bouderius、红河长臀C.henrici产生了一定的遗传分化,而珠江和红河群体未发现明显遗传分化,从遗传距离来看,珠江和红河长臀净遗传距离为0.000。【结论】长臀野生资源较为贫乏,且海南群体最为严重;认为应将珠江长臀和红河长臀归为同一亚种长臀C.bouderius,而海南长臀作为长臀的另一个亚种。【Objective】In order to understand the current situation of wild resources for three Cranoglanis populations and to evaluate the species validities of three species.【Method】The genetic structure and genetic variation of three Cranoglanis populations（ C. multiradiatus,C. bouderius and C. henrici） were investigated by sequencing the Dloop genes in the mitochondrial control region. 【Result】Among 84 individuals from the three populations,fourty-three hapotypes were detected,indicating a relatively high haplotype diversity and low nucleotide diversity. The lowest hapotype diversity（ Hd） and nucleotide diversity（ Pi） were both found in C. multiradiatus group（ Hd = 0. 871,Pi = 0. 006 4）. Tajima＇s D neutral test and nucleotide mismatch distribution analysis showed that the three populations were getting stable without experincing any large-scale expansion. Fst analysis suggested that C. multiradiatus had certain genetic differentiation compared to C. bouderius and C. henrici,while the latter two did not have any obvious differentiation from each other. The genetic distance between C. bouderius and C. henrici was 0. 000.【Conclusion】The wild resources for Cranoglanis are relatively poor and the case in C. multiradiatus is the most serious. C. bouderius and C. henrici are considered to be one subspecies,as well as C. multi-radiatus being another subspecies of Cranoglanis.

关 键 词：长臀 线粒体控制区 Dloop基因序列 物种有效性 遗传结构 资源状况 

分 类 号：Q953[生物学—动物学]