黄梁木种源抗寒性综合评价  

Comprehensive evaluation of cold resistance of Neolamarckia cadamba

作　　者：黄浩[1,2] 周祥斌[2] 欧阳昆唏[2] 李俊成[2] 曾昭佳 陈晓阳[2] 

机构地区：[1]广西药用植物园,广西南宁530023 [2]华南农业大学林学与风景园林学院/亚热带农业生物资源保护与利用国家重点实验室/广东省森林植物种质创新与利用重点实验室,广东广州510642 [3]广东省九连山林场,广东河源517100

出　　处：《华南农业大学学报》2016年第1期82-88,共7页Journal of South China Agricultural University

基　　金：国家林业局林业公益性行业科研专项(201004020)

摘　　要：【目的】为黄梁木Neolamarckia cadamba抗寒选育奠定理论基础和人工种植提供技术支持。【方法】对7个不同黄梁木种源带芽枝条进行人工低温胁迫,测定丙二醛、游离脯氨酸、可溶性糖、可溶性蛋白质含量、相对电导率和枝条恢复生长情况。确定适用于黄梁木抗寒性测定的5个指标,应用主成分分析和隶属函数法对抗寒性进行综合评判。【结果】在-2℃的人工低温胁迫下,丙二醛质量分数最高的种源为龙州(35.24μg·g-1),最低的种源为保山(21.19μg·g-1);游离脯氨酸质量分数最高的种源为龙州(9.98μg·g-1),最低的种源为德宏(6.13μg·g-1);可溶性糖质量分数最高的种源为云浮(79.66μg·g-1),最低的种源为景洪(65.32μg·g-1);可溶性蛋白质质量分数最高的种源为天河(24.89μg·g-1),最低的种源为景洪(19.57μg·g-1);相对电导率最大的种源为保山(58.27%),最小的为云浮(48.37%);在枝条恢复生长中,存活率最高的种源为云浮(42.03%),最低的为景洪(3.88%)。云浮、龙州、天河、兴宁、景洪、保山、德宏7个种源的抗寒性综合指数大小依次为0.951、0.863、0.755、0.728、0.191、0.151、0.132。【结论】不同种源抗寒性从强到弱依次为云浮、龙州、天河、兴宁、景洪、保山、德宏,总体表现为广东、广西的种源抗寒性较云南种源强。【Objective】This study aimed to lay a theoretical foundation for cold resistance breeding and to provide a technical support for artificial cultivation of Neolamarckia cadamba. 【Method】After the branches of seven provenances were treated with artificially induced low temperature,the contents of methane dicarboxylic aldehyde（ MDA）,free proline,soluble sugar and soluble protein,the relative electrical conductivity（ REC） as well as the branch recovery growth were measured. Using five indicators suitable for determination of cold resistance,combined with principal component analysis and membership function method,the cold resistance of different provenances was comprehensively evaluated. 【Result】Under artificially induced low temperature stress（- 2 ℃）,the Longzhou provenance had the highest MDA content（ 35. 24 μg·g- 1） and Baoshan had the lowest（ 21. 19 μg·g- 1）; Longzhou had the highest proline content（ 9. 98 μg·g- 1） and Dehong had the lowest（ 6. 13 μg·g- 1）; Yunfu had the highest content of solu-ble sugar（ 79. 66 μg·g- 1） and Jinghong had the lowest（ 65. 32 μg·g- 1）; Tianhe had the highest content of soluble protein（ 24. 89 μg·g- 1） and Jinghong had the lowest（ 19. 57 μg·g- 1）; Baoshan had the highest REC（ 58. 27%） and Yunfu had the lowest（ 48. 37%）; During the branch recovery growth,Yunfu had the highest branch bud survival rate（ 42. 03%） and Jinghong had the lowest（ 3. 88%）. The integrated indices of cold resistance for seven provenances,Yunfu,Longzhou,Tianhe,Xingning,Jinghong,Baoshan and Dehong were 0. 951,0. 863,0. 755,0. 728,0. 191,0. 151 and 0. 132 respectively.【Conclusion】The cold resistance of Yunfu provenance was the strongest,followed by Longzhou,Tianhe,Xingning,Jinghong,Baoshan and Dehong. In general,the cold resistance of Guangdong or Guangxi provenance was stronger than that of Yunnan provenance.

关 键 词：种源 黄梁木 抗寒性 隶属函数法 主成分分析 

分 类 号：S722.5[农业科学—林木遗传育种]