大鼠胃旁路手术模型建立及影响因素  

Establishment of gastric bypass surgery model on rats and influence factors

作　　者：曾松华[1] 吴良平[2] 戴晓江[2] 张红兵[2] 黄永滔[2] 黎飞[1] 李海贞[2] 张玉新[2] 

机构地区：[1]南方医科大学,广东广州510515 [2]广州军区广州总医院甲状腺糖尿病外科中心,广东广州510010

出　　处：《局解手术学杂志》2016年第1期5-8,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：广东省科技计划项目(2011B050400041);广东省自然科学基金资助项目(S2011010000472)

摘　　要：目的建立SD大鼠胃旁路手术模型,分析SD大鼠胃旁路手术的影响因素及其预防措施,为进一步建立糖尿病大鼠(GK大鼠)胃旁路手术模型提供经验和技术积累。方法对30只SD大鼠实施胃旁路手术,分析影响建模成败的各种因素。结果大鼠建模成功17只,死亡13只,死亡率43.3%。3只大鼠在术中死亡,其中麻醉因素致死2只,心衰致死1只;1只因吻合口出血在术后2 h死亡;8只在术后第3天死亡,其中感染致死2只,小肠坏死致死2只,吻合口狭窄或梗阻致死4只;1只大鼠在术后第5天因误食固体食物致吻合口瘘死亡。本组大鼠无切口裂开等其他并发症。结论 SD大鼠胃旁路手术模型作为GK大鼠胃旁路手术模型的预实验是经济可行的。麻醉及围手术期管理、手术技巧、感染防治是建模成败的主要影响因素,加强相关因素的控制可以提高建模成功率。Objective To establish gastric bypass surgery model with SD rat and analyze the influence factors and preventive measures so as to accumulate experience and skills for further developing gastric bypass surgery model on diabetic rat（ GK rat）. Methods Gastric bypass surgery was operated on 30 SD rats. Summarized modeling experience and analyzed various factors affecting modeling success. Results17 rats weresuccessfully survived after surgery and 13 failed,with the mortality rate 43. 3%. Three rats died during operation,including two for anesthesia and one for heart failure; one died due to anastomotic bleeding two hours after surgery. Eight died in the third day after surgery,including two for infection,two for intestinal necrosis,four for anastomotic stenosis or obstruction. One died because of anastomotic leakage caused by mistaking and satiety the fifth day after surgery. There were no other complications such as wound dehiscence occurred in this group. Conclusion Establishing models of gastric bypass surgery with SD rats which is regarded as pre-experiment of building models of gastric bypass surgery with GK rats is economical and feasible. Anesthesia and perioperative management,surgical techniques and infection prevention and control are the main factors affecting modeling. The survival rate of rat model could be increased if related factors well controlled.

关 键 词：SD大鼠 胃旁路手术 动物模型 

分 类 号：R656.6[医药卫生—外科学] R587.1[医药卫生—临床医学]