外源亚精胺对盐胁迫下大豆幼苗叶片光合性能和抗氧化代谢的调控效应  

Regulating Effects of Exogenous Spermidine on Photosynthetic Character and Antioxidant Metabolism of Soybean Seedling Leaves under Salt Stress

作　　者：杜红阳[1,2] 胡春红[1] 刘怀攀[1] 

机构地区：[1]周口师范学院生命科学与农学学院/植物遗传与分子育种重点实验室,河南周口466001 [2]河南农业大学农学院,河南郑州450003

出　　处：《大豆科学》2016年第1期80-85,共6页Soybean Science

基　　金：国家公益性行业(农业)专项(201203077);国家自然科学基金(31271627);河南省基础与前沿技术研究计划(082300430310)

摘　　要：为了探讨亚精胺提高大豆幼苗抗盐性的内在机理,以郑豆30为材料,采用沙培法,研究了在100 mmol·L^(-1)盐胁迫下,不同浓度(0.1,0.5,1.0和1.5 mmol·L^(-1))的亚精胺对大豆幼苗叶片光合特性和抗氧化代谢的影响。结果表明:在盐胁迫下,大豆幼苗相对干物质增长速率(RDMIR)、抗氧化酶(SOD、POD、CAT和APX)活性和光合参数(Pn、Ci、Tr和Gs)下降,相对电解质渗透率(RELR)、超氧阴离子产生速率(O^-_2·)、过氧化氢(H_2O_2)和丙二醛(MDA)含量上升,但经过不同浓度的亚精胺处理后,大豆幼苗叶片RELR、O^-_2·、H_2O_2和MDA含量下降,而RDMIR、抗氧化酶活性和光合参数却上升,且以0.5 mmol·L^(-1)的Spd处理效果最显著。这些结果表明亚精胺能通过提高大豆幼苗叶片的抗氧化酶活性而降低幼苗叶片的O^-_2·产生速率、H_2O_2和MDA含量,通过提高光合相关参数指标提高幼苗叶片的光合效率,从而减轻盐胁迫的伤害,促进大豆幼苗生长。In order to explore the mechanism that spermidine（Spd） improves soybean seedlings resistance to salt stress, takeing Zhengdou 30 as material, we studied the effects of Spd with different concentration on photosynthetic character and antioxidant metabolism of soybean seedling leaves under salt stress（100 mmol.L-1NaCl） The results are as follows： under salt stress, the relative drought matter increase rate （RDMTR）, the activity of antioxidant enzymes （superoxide dismutase： SOD, peroxidase： POD, catalase： CAT, and ascorbic acid oxidase ： APX） and photosynthetic parameters （net photosynthetic rate： Pn, stomatal conductance： Gs, intercellular CO2？concentration： Ci, transpiration rate：Tr） of soybean seedling leaves significantly decreased, however, relative electrolyte leakage rate （RELR）, superoxide anion generation rate（O-2） , hydrogen peroxide（H2O2）and malondialdehyde（MDA）contents increased. But, exogenous Spd with different concentration increased RDMIR, the activity of antioxidant enzymes and photosynthetic parameters of soybean seedling leaves and decreased RELR ,O -2, H2O2and MDA contents, and the increases or decreases are markedly at 0.5mmol.L-1Spd concentration. These results suggest that the appropriate concentration of Spd could decrease O-2generation rate and the contents of H2O2and MDA via increasing the activities of antioxidant enzymes in soybean seedlings and increase photosynthetic efficiency via improving photosynthetic parameters to alleviate the injury of salt stress to soybean seedlings and promote the growth of soybean seedlings.

关 键 词：亚精胺 盐胁迫 大豆幼苗 光合性能 抗氧化 

分 类 号：S565.1[农业科学—作物学]