慢性HBV感染HBsAg含量与T、B淋巴细胞亚群及NK细胞相关性  

Association between HBsAg levels and T,B lymphocyte subsets or NK cells in chronic HBV infected patients

作　　者：聂琳[1] 杨丽莎[2] 彭德珍[3] 张敏[1] 王君宜 

机构地区：[1]桂林医学院附属医院消化内科,桂林541001 [2]桂林医学院附属医院中医科,桂林541001 [3]桂林医学院附属医院检验科,桂林541001

出　　处：《安徽医科大学学报》2016年第1期82-85,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81260602)

摘　　要：目的探讨慢性乙型肝炎病毒(HBV)感染免疫不全状态(非活动期)乙型肝炎表面抗原(HBs Ag)含量与外周血T、B淋巴细胞亚群及NK细胞的相关性。方法收集慢性HBV感染免疫不全状态(非活动期)者34例、健康者20例,采用流式细胞仪检测其外周血T、B淋巴细胞亚群及NK细胞百分比,实时荧光探针定量-聚合酶链反应(FQ-PCR)方法检测HBs Ag的含量。结果 HBs Ag的含量与B淋巴细胞百分比呈正相关性(P<0.05),高HBs Ag含量组的B淋巴细胞高于健康组与低HBs Ag含量组,差异有统计学意义(P<0.05),与T淋巴细胞及NK细胞无相关性。结论在慢性HBV感染免疫不全状态(非活动期)的人群中,HBs Ag含量在一定程度上能反映患者的免疫状态,外周血淋巴细胞亚群和NK细胞检测对于了解患者免疫状态有一定的临床价值。Objective To investigate the correlation between the levels of HBsAg and T, B and NK cells subgroup in immune incompetence （ inactive phase） which were infected by HBV. Methods Thirty four cases of immunodefi- ciency （inactive phase） which were infected by HBV were selected in our study, at the same time, twenty normal cases for control. The levels of peripheral blood lymphocyte subsets and NK cells were detected by the flow cytome- try. Serum HBsAg levels were tested by Real-time fluorescent probe quantitative polymerase chain reaction （FQ- PCR）. Results The content of HBsAg was positively correlated with the percentage of B lymphocytes in inactive state cases. The levels of B lymphocytes in the group of high content of HBsAg was higher than that in the healthy group as well as the group of low content of HBsAg （P 〈 0. 05 ） . However, there was no statistically significant correlation between it and T, B or NK cells. Conclusion Among patients which were infected by HBV in inactive phase, the serum HBsAg levels could represent B cells levels to some extent. The detection of peripheral blood lym- phocyte subsets and NK cells have clinical value for understanding the immune status of patients.

关 键 词：乙肝病毒表面抗原含量 T淋巴细胞 B淋巴细胞 自然杀伤细胞 免疫不全状态(非活动期) 

分 类 号：R446.11[医药卫生—诊断学] R373.2[医药卫生—临床医学]