HPLC-ELSD法同时测定不同产地白果药材中4种萜内酯类成分  

Simultaneous determination of four terpene lactones in Ginkgo Semen from different growing areas by HPLC-ELSD

作　　者：张群群[1] 李慧芬[1,2] 张学兰[1,2] 吴鹏[1] 王均秀 宋梦晗 赵鑫[1] 

机构地区：[1]山东中医药大学,山东济南250355 [2]国家中医药管理局中药炮制传承基地,山东济南250355

出　　处：《中成药》2016年第1期133-136,共4页Chinese Traditional Patent Medicine

基　　金：济南市高校自主创新计划项目(201303027)

摘　　要：目的建立HPLC-ELSD法同时测定白果药材中白果内酯和银杏内酯A、B、C。方法白果乙酸乙酯萃取物的分析采用Kromasil C_（18）色谱柱（250 mm×4.6 mm,5μm）;流动相为甲醇-0.1%甲酸水（38∶62）;柱温30℃,体积流量0.8 m L/min。结果白果内酯和银杏内酯A、B、C分别在1.01~40.40μg、1.03~41.20μg、2.37~94.80μg、1.51~60.40μg范围内线性关系良好（r〉0.999 5）,平均回收率在97.3%~99.2%之间。5个不同产地（山东济南、临沂、泰安,广西桂林和江苏泰兴）白果药材中四者的含有量分别为75.55~219.73、54.79~135.88、200.37~853.39、119.31~498.06μg/g。结论白果中4种萜内酯类成分的总含有量以江苏省泰兴市产者为最高。AIM To establish a method for simultaneously determining the contents of bilobalide and ginkgolide A,B,C in Ginkgo Semen by HPLC-ELSD. METHODS The analysis of Ginkgo Semen ethyl acetate extract was performed on Kromasil C_（18） column（ 250 mm × 4. 6 mm,5 μm）,mobile phase was methanol-0. 1% methanoic acid water（ 38 ∶ 62）,column temperature was maintained at 30 ℃,and flow rate was 0. 8 m L / min. RESULTS Bilobalide and ginkgolide A,B,C had good linear relationships in the ranges of 1. 01- 40. 40 μg,1. 03-41. 20 μg,2. 37- 94. 80 μg and 1. 51- 60. 40 μg,respectively（ r 0. 999 5）. Their average recoveries were97. 3%- 99. 2%. The contents of these four consitituents in Ginkgo Semen from five different growing areas（ Jinan,Linyi,Taian in Shandong,Guilin in Guangxi and Taixing in Jiangsu） were 75. 55- 219. 73 μg / g,54. 79-135. 88 μg / g,200. 37- 853. 39 μg / g and 119. 31- 498. 06 μg / g,respectively. CONCLUSION The total contents of four terpene lactones in Ginkgo Semen is the highest in Taixing（ Jiangsu）.

关 键 词：白果 白果内酯 银杏内酯A 银杏内酯B 银杏内酯C 产地 HPLC-ELSD 

分 类 号：R284.1[医药卫生—中药学]