磷酸钙相组成对类骨单位复合骨支架的影响（英文）  

Effect of Phase Composition of Calcium Phosphate(CaP) on Bioactivity of Osteon-like Composite Scaffolds

作　　者：陈雪宁[1] 范红松[1] 王红军 

机构地区：[1]四川大学国家生物医学材料工程技术研究中心,成都610064 [2]斯蒂文斯理工学院化学、生化和生物医学工程学院,美国新泽西07030

出　　处：《无机材料学报》2016年第1期107-112,共6页Journal of Inorganic Materials

基　　金：National Science Foundation of China(31400819);National Science Foundation of USA(NSF,DMR-0922522)

摘　　要：从组成上看,自然骨是一种无机与有机的复合材料;从结构上看,致密骨的基本结构单位为内壁血管化的骨单位。本研究基于组成与结构仿生的原理制备组织工程化支架,模拟具有复杂结构的密质骨的基本单位——骨单位。为此,通过静电纺丝和双螺杆挤出相结合的两步制造法,制备一种具有双层结构的聚己内酯/磷酸钙(PCL/Ca P)复合骨支架,其内层是由电纺纳米纤维组成的空心管,可贴附内皮细胞层形成与哈佛氏管相类似的结构;其外层是具有高孔隙率的螺旋状PCL/Ca P微丝,可复合前成骨细胞以模拟骨单位结构中的外层骨样组织。为进一步探索材料组成对于支架生物功能的影响,分别设计了外层为PCL,PCL/双相磷酸钙(PCL/BCP)和PCL/?-磷酸三钙(PCL/?-TCP)的复合支架,比较了材料组分变化对外层微丝结构及前成骨细胞(MC3T3-E1)活性的影响。相比于PCL和PCL/?-TCP,PCL/BCP微丝更能显著增强细胞的生长和钙的沉积,并成功获得可精确调控不同细胞的空间分布的双层复合支架,实现对复杂结构骨单位的模拟构建,显示出很好的应用前景。From compositional perspective, natural bone is a kind of inorganic and organic composite material; while from structural perspective, the basic structural unit of cortical bone is vascularized osteon. In this study, based on the biomimetic principle, a tissue-engineered construct was created to stimulate the basic building block of cortical bone-osteon with complex structure. To achieve this goal, a bi-layered polycaprolactone （PCL）/CaP com- posite scaffold was produced via a novel two-step fabrication process in the combination of electrospinning and twin screw extrusion. Its inner hollow tube was made of electrospun nanofibers, allowing the formation of a con- fluent layer of endothelial cells （MS-I） to resemble the Haversian canal, and its outer layer consisted of spiral PCL/CaP microfilament with high porosity, facilitating the proliferation and differentiation of pre-osteoblasts （MC3T3-E1） to form bone-like tissues. To explore the effect of material composition on scaffold bioactivity, three composite scaffolds with different outer layers were fabricated, including PCL, PCL/biphasic calcium phosphate （BCP） and PCL/β-tricaclium phosphate （β-TCP）. And then the influences of scaffold composition on the behaviors of pre-osteoblasts were further investigated. Compared to PCL and PCL/β-TCP, significantly more cell growth and higher calcium deposition are found on PCL/BCP scaffolds, precisely controlling the spatial distribution of different cells. Taken together, this bi-layered PCL/BCP scaffold partially mimics the complex structure of osteon, showing a promising potential in the orthopedic application.

关 键 词：磷酸钙 相组成 复合材料 骨单位 双螺杆挤出 

分 类 号：R318[医药卫生—生物医学工程]