IVF-ET周期中1原核(PN)及0PN(2Pb)胚胎的染色体组成分析  

Analysis of the chromosome composition of embryos exhibiting non- and mono-pronuclear at the fertilization check in in vitro fertilization cycles

作　　者：张亚楠[1,2] 殷宝莉[2] 张翠莲[2] 谢娟珂[2] 韦多[2] 宋小兵[2] 刘琦[2] 

机构地区：[1]郑州大学人民医院,郑州450003 [2]河南省人民医院,郑州450003

出　　处：《生殖与避孕》2016年第1期26-31,共6页Reproduction and Contraception

基　　金：河南省科技厅重点科技攻关项目(No.122102310536);河南省科技厅科技攻关项目(No.132106000081)

摘　　要：目的:初步探寻体外受精-胚胎移植(IVF-ET)周期中高发育潜能的1原核(PN)及0PN(2Pb)胚胎的方法。方法:观察787个IVF周期患者的PN及卵裂情况,培养至第6日,观察其囊胚形成情况。取囊胚滋养层细胞活检,采用胚胎植入前遗传学筛查(PGS)技术检测0PN(2Pb)、1PN及2PN囊胚的染色体组成。结果:787个IVF周期共获卵8 352枚,2PN、0PN(2Pb)、1PN胚胎的形成率分别为64.35%、4.93%、3.65%,囊胚形成率分别为45.71%、30.03%、18.18%。PGS结果显示,1PN及0PN(2Pb)囊胚染色体正常的比例明显低于2PN囊胚,差异有统计学意义(P<0.05)。0PN(2Pb)囊胚的染色体正常比例较1PN囊胚的略高,差异无统计学意义(P>0.05)。此外,发育至第6日的0PN(2Pb)囊胚及1PN囊胚较第5日胚胎的正常染色体比例高。结论:对于本周期无可利用胚胎的患者,建议移植1PN及0PN(2Pb)来源的第6日评分较好的囊胚。Objective： To find out a good method to select the highest development potential embryos exhibiting non- and mono-pronuclear （PN） at the fertilization check. Methods： The pronuclear zygote morphology and cleavage of 788 IVF cycles were evaluated, which subsequently cultured to day 6. Then the chromosomal constitution of blastocysts derived from non- and mono-pronuclear was checked by trophectoderm cell biopsy and preimplantation genetic screening （PGS）. Results： In the 788 IVF cycles, 8 352 oocytes were retrieved, of which 64.35% developed to bi-pronuclear zygotes and the other 4.93% and 3.65% were non- and mono-pronuclear zygotes respectively. Incidences of blastocysts for bi-, non- and mono-pronuclear zygotes were 45.71%, 30.03% and 18.18%, respectively. The array-based comparative genomic hybridization （aCGH） results showed that the percentage of normal chromosomal composition of blastocysts from non- and mono- pronuclear zygotes was significantly lower than that of blastocysts from bi-pronuclear zygotes, while it was slightly higher in the non-pronuclear ones than in the mono-pronuclear ones. Furthermore, the percentage of normal chromosomal composition of blastocysts derived from non- and mono-pronuclear zygotes was higher on day 6 than on day 5. Conclusion： The results demonstrated that blastocysts derived from non- and mono- pronuclear zygotes in IVF cycles with good morphology on day 6 might be utilized for embryo transfer for patients without enough available embryos.

关 键 词：体外受精(IVF) 1原核(PN)胚胎 0PN(2Pb)胚胎 胚胎植入前遗传学筛查(PGS) 染色体组成 

分 类 号：R714.8[医药卫生—妇产科学]