LPS通过TLR4影响绵羊输卵管上皮细胞SBD-1的表达  

LPS Induces SBD-1 Expression via TLR4 in Ovine Oviduct Epithelial Cells

作　　者：李琦[1] 智达夫[1] 延沁 刘默宁 郑欣欣[1] 曹贵方[1] 

机构地区：[1]内蒙古农业大学兽医学院动物组织胚胎与发育生物学研究室,呼和浩特010018

出　　处：《畜牧兽医学报》2016年第1期79-84,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(31360593;31060328);内蒙古自治区科技计划项目(20130317)

摘　　要：旨在研究脂多糖(LPS)对绵羊输卵管上皮细胞β-防御素-1(SBD-1)表达的影响及其调控途径,本研究设置不同浓度(10ng·mL-1、50ng·mL-1、100ng·mL-1、200ng·mL-1、1mg·mL-1)LPS,分别作用不同时间(0、1、3、6、12和24h),检测绵羊输卵管上皮细胞SBD-1和Toll样受体-4(TLR4)mRNA表达水平,通过免疫组化检测SBD-1表达定位,并进一步通过TLR4阻断试验来证实TLR4介导LPS引起SBD-1表达。结果表明,SBD-1蛋白表达于输卵管上皮细胞。LPS以浓度和时间依赖方式影响绵羊输卵管上皮细胞中SBD-1的表达,且100ng·mL-1 LPS在作用12h后,SBD-1的表达水平达到最高。阻断试验表明,TLR4介导LPS对SBD-1的表达调控。综上表明,LPS可以影响绵羊输卵管上皮细胞表达SBD-1,且该过程通过TLR4介导。The aim of this study was to explore the effect of lipopolyscharride（LPS）on induction of sheep beta defensin-1（SBD-1）in ovine oviduct epithelial cells（OOECs）.In this study,OOECs were cultured and then exposed to LPS for up to 24 hours,with a range of concentrations（10ng·mL^-1-1mg·mL^-1）.qRT-PCR was performed to detect SBD-1mRNA.The results showed that LPS stimulated the expression of SBD-1in a time-（maximal at 12hours）and concentration-（maximal at 100ng·mL^-1）dependent manner.Moreover,IHC staining showed that SBD-1was mainly expressed in epithelial cells.Importantly,TLR4 was detected in OOECs and stimulated by LPS.LPS induced SBD-1mRNA expression was markedly inhibited by blockage of TLR4 activity using anti-TLR4 antibody.In conclusion,these results suggest that LPS might induce the expression of SBD-1in the ovine oviduct epithelial cells,and the expression are mediated by TLR4.

关 键 词：输卵管 脂多糖 绵羊β-防御素-1 TOLL样受体-4 

分 类 号：S826.2[农业科学—畜牧学]