马立克病病毒与禽网状内皮增生病病毒的混合感染及在传代过程中的重组  

Co-infection of Field Marek's Disease Virus with Reticuloendotheliosis Virus Prevalent in Vaccinated Chicken Flocks

作　　者：宿靖伟[1,2] 滕蔓[1] 罗俊[1,3] 迟佳琦[1] 余祖华[3] 禹乐乐[1] 胡博[1] 张改平[1,2] 

机构地区：[1]河南省农业科学院动物免疫学重点实验室,农业部动物免疫学重点实验室,河南省动物免疫学重点实验室,郑州450002 [2]河南农业大学牧医工程学院,郑州450002 [3]河南科技大学动物科技学院,动物疫病与公共安全重点实验室,洛阳471003

出　　处：《畜牧兽医学报》2016年第1期128-134,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31372445);NSFC-广东省政府联合基金(U1131005);河南省农业科学院优秀青年科技基金(2013YQ28)

摘　　要：旨在了解国内鸡群马立克病病毒(MDV)和禽网状内皮增生病病毒(REV)的混合感染及自然重组状况,探讨两种病原的基因重组对MDV病原学特性的潜在影响及危害。利用PCR扩增、序列测定及间接免疫荧光试验(IFA),对来自河南商品蛋鸡群的17个MDV分离株进行研究。结果表明,其中两个毒株HNGS206和HNXZ103的病毒基因组中存在REV—LTR的重组插入。进一步的IFA结果显示,HNGS206和HNXZ103并非MDV与REV的自然重组流行毒株,这两个毒株基因组中LTR的插入发生于临床病例鸡混合感染后的病毒分离及传代培养过程中。结果提示,虽然目前国内鸡群中MDV和REV的混合感染较为普遍,但MDV与REV自然重组毒株的流行状况仍需要进一步的流行病学监测。This study was performed to investigate the co-infection and natural recombination of Marek＇s disease virus（MDV）and reticuloendotheliosis virus（REV）in chicken flocks in China,and evaluate the potential influences of viral recombination on MDV biology.Based on polymerase chain reaction（PCR）,DNA sequencing and indirect immunofluorescent assay（IFA）,we presently studied a total of 17 MDV field isolates obtained from the vaccinated chicken flocks in Henan province.The PCR results showed that,among the 17 Henan isolates,two isolates,HNGS206 and HNXZ103,are recombinant MDVs containing the REV-LTR inserts.Subsequent experiments showed that neither of the two isolates are natural recombinant viruses and the integrations of LTR into the viral genomes may result from the clinical co-infection and/or virus isolation procedure.Our data indicates that although a wide-spread co-infection of both MDV and REV exists inchicken flocks in China,the epidemiology of the naturally occurring recombinant MDVs needs to be further monitored.

关 键 词：马立克病病毒 禽网状内皮增生病病毒 流行病学 混合感染 病毒传代 基因重组 

分 类 号：S858.315.3[农业科学—临床兽医学]