基于核磁共振技术的卵巢静止奶牛血浆代谢谱分析  

1 H-NMR-based Plasma Metabolic Profiling of Postpartum Dairy Cows with Ovarian Inactivity

作　　者：许楚楚[1] 夏成[1] 孙雨航[1] 肖鑫焕 王刚[1] 舒适[1] 张洪友[1] 徐闯[1] 杨威[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319

出　　处：《畜牧兽医学报》2016年第1期190-197,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然基金面上项目(31372488);国家科技支撑计划课题(2013BAD21B01-2;2012BAD12B05;2013BAD21B00);黑龙江八一农垦大学研究生创新科研项目(YJSCX2015-Y22)

摘　　要：为研究卵巢静止奶牛与发情奶牛体内差异代谢物变化,选取产后60-90d的年龄、胎次、体况相近的健康经产高产奶牛作为实验动物。根据奶牛发情表现、直肠检查和B超检查及激素检测的结果,选取10头奶牛为发情组（A）,10头奶牛为卵巢静止组（B）,然后对两组奶牛血浆样品进行无偏性的1 H谱核磁共振检测,对比其血浆代谢组学图谱,进行多元统计分析,筛选及鉴定卵巢静止奶牛血浆差异代谢产物,并进行生物信息学分析。结果显示：相对于正常发情奶牛,卵巢静止奶牛血浆中共12种代谢产物表现异常,包括水平增加的乙酸、柠檬酸和酪氨酸,以及水平下降的低密度脂蛋白、极低密度脂蛋白、脂质、丙氨酸、丙酮酸、肌酸、胆碱、磷酸胆碱和甘油磷酸胆碱等;这些代谢产物与糖代谢、氨基酸代谢、脂蛋白代谢和胆碱代谢的异常密切相关;通过参与生物合成通路调节,干扰了奶牛正常卵泡生长。并且发现卵巢静止的奶牛血浆肌酸浓度降低和酪氨酸浓度升高的新变化。多种代谢异常提示奶牛卵巢静止与泌乳早期能量负平衡有关,并有伴发酮病和脂肪肝病的风险;另外,卵巢静止奶牛血浆肌酸、胆碱浓度降低和酪氨酸浓度升高的变化,为今后更为深入研究奶牛卵巢静止发生机制提供了新的方向。To understand the differences in metabolic changes between cows with ovarian inactivity and estrus cows,we selected cows at 60-90 dpostpartum,which had similar age,parity,body condition score and milk yield.According to clinical manifestations,B-ultrasound scan,rectal examination and hormone tests,10 cows were assigned to the estrus group（A）and 10 to the ovarian inactivity group（B）.All plasma samples were analyzed by ^1 H NMR spectroscopy to compare plasma metabolomic profiles between the groups.We used multivariate pattern recognition,to screen for different metabolites in plasma of anestrus cows.Compared with normal estrous cows,there were abnormalities in 12 kinds of metabolites in postpartum cows with ovarian inactivity,including an increase in acetic acid,citric acid and tyrosine,and a decrease in low-density lipoprotein,very low density lipoprotein,lipids,alanine,pyruvate,creatine,choline,phosphorylcholine and glycerophosphorylcholine.These metabolites were closely related to abnormality of glucose,amino acid,lipoprotein and choline metabolism,which may disturb the normal estrus.The decrease in plasma creatine and the increase in tyrosine were new changes for ovarian inactivity of postpartumcows.In conclusion,a variety of metabolic disorders indicate that ovarian inactivity is related to negative energy balance in early lactation,and has the risk of ketosis and fatty liver.The decrease of creatine and choline concentration and increase of p-hydroxyphenylalanine concentration in cows with ovarian inactivity provide a new direction for research on the mechanism of ovarian inactivity in cows.

关 键 词：奶牛 卵巢静止 核磁共振技术 血浆差异代谢物 

分 类 号：S857.22[农业科学—临床兽医学]