GEFH1在LPS诱导树突细胞IL-6和IL-12a表达中的作用  

Effect of CEFH1 on the expression of IL-6 and IL-12 in LPS-induced dendritic cells

作　　者：唐蓓[1] 李影[1] 

机构地区：[1]重庆师范大学生命科学学院,重庆401331

出　　处：《现代免疫学》2016年第1期32-35,共4页Current Immunology

基　　金：重庆市自然科学基金(cstc2014jcyjA10020)

摘　　要：为探讨GEFH1与树突细胞(dendritic cell,DC)TLR4两条下游信号途径的关系,从野生型和TRIF基因、IFNα/β受体基因敲除小鼠中分离和培养DC,LPS或IL-6刺激后收集细胞制备总cDNA,通过实时定量PCR检测GEFH1的mRNA表达。再用GEFH1的siRNA转染DC,LPS刺激后检测IL-6和IL-12a的mRNA表达。结果,在LPS刺激后,GEFH1的mRNA表达在野生型小鼠的DC中显著增加,在TRIF基因、IFN-α/β受体基因敲除小鼠的DC中则未被上调。此外,GEFH1siRNA处理后,IL-6和IL-12a的mRNA表达均上升显著(P>0.05),而在IL-6刺激的野生型小鼠的DC中,GEFH1的mRNA没有明显改变。以上结果提示在转录水平,TRIF-IFNβ信号通路、而非IL-6可诱导GEFH1基因表达。GEFH1可能对MyD88途径中细胞因子IL-6和IL-12a的表达有负调节作用。To elucidate the crosstalk between GEFH1 and the two downstream pathways of TLR4 signaling in dendritic cells （DCs）, we used Real-time PCR to detect GEFH1 mRNA levels in LPS or IL-6 stimulated DCs isolated from wild-type （WT） mice,TRIF knockout （TRIFKO）mice and IFN-α/β receptor knockout（IFN-α/βRKO） mice respectively. We also analyzed IL-6 and IL-12a rnRNA expression in DCs after GEFH1 was silenced by siRNA. The results showed that up-regulation of GEFH1 mRNA was observed in DC from WT mice but not in DC from TRIFKO and IFN-α/βRKO mice after LPS incubation, indicating that LPS-induced up-regulation of GEFH1 was attenuated by knockout of IFN-α/βreceptor. In addition, GEFH1 siRNA alter IL-6 and IL-12a mRNA levels in DC after LPS stimulation. But failed to alter GEFH1 mRNA level in DC after IL-6 stimulation. In conclusion, GEFH1 expression has been proved to be related to the TRIF- IFNβ pathway. GEFH1 may be have negative effecton the expression of IL-6 and IL-12a in the MyD88 pathway, while there is no evidence that GEFH1 is involved in IL- 6 signal pathway.

关 键 词：GEFH1 TLR4信号途径 TRIF MYD88 

分 类 号：R392[医药卫生—免疫学]