前列腺液中IL-8浓度与前列腺增生组织中bFGF、Bcl-2表达的相关性  

Relationship between interleukin-8 levels in expressed prostatic secretion and expressions of bFGF and Bcl-2 in benign prostatic hyperplasia

作　　者：宋武[1] 李冬阳[2] 袁海川[1] 顾朝辉[1] 邵佳亮[2] 王翔[2] 

机构地区：[1]复旦大学附属华山医院宝山分院泌尿外科,上海201900 [2]复旦大学附属华山医院泌尿外科

出　　处：《中华医学杂志》2016年第2期104-107,共4页National Medical Journal of China

摘　　要：目的观察前列腺液中白细胞介素（IL）-8浓度与前列腺增生组织中碱性成纤维细胞生长因子（bFGF）、B细胞淋巴因子2（Bcl-2）表达的相关性。方法收集50例经尿道前列腺电切术的良性前列腺增生（BPH）患者的术前前列腺液标本及术中前列腺组织标本。根据组织病理学特征确定是否合并前列腺炎症（CP），将标本分为单纯性BPH组和BPH合并CP组。采用酶联免疫吸附试验（ELISA法）检测前列腺液中IL-8浓度，采用免疫组化法检测bFGF、Bcl-2在前列腺组织中的表达情况。结果（1）组织病理学研究发现，50例TURP切除的前列腺组织标本中，有30例（60％）患者前列腺组织中存在炎症浸润。BPH合并CP组的前列腺体积显著大于单纯性BPH组[（58．9±7．2）与（44．9±2．4）ml]（P=0．022），并且慢性前列腺炎症状评分（NIH—CPSI）（24．1±8．2）和国际前列腺症状评分（IPSS）（24．6±6．5）也显著高于单纯性BPH组（11．8±5．6、17．3±4．1）（P〈0．05）；（2）IL-8、Bcl-2和bFGF在两组中均有表达，且这3项指标在BPH合并CP组中浓度均明显高于单纯性BPH组（P〈0．05）；（3）两组中IL-8的浓度与Bcl-2及bFGF的浓度分别呈正相关性（0〈R〈1，P〈0．05）。结论前列腺炎症与前列腺增生发生发展相关并且可导致前列腺增生更加严重。Objectives To investigate the relationship between interleukin-8 levels in expressed prostatic secretion and expressions of basic fibroblast growth factor （bFGF） and Bcl-2 in benign prostatic hyperplasia （BPH）. Methods A series of 50 consecutive patients diagnosed with BPH and scheduled for transurethral resection of the prostate （TURP） were included. Patients were divided into two groups, simple BPH and BPH with chronic prostatitis （CP）. The grade of inflammatory changes in the prostate was then determined blindly by two experienced pathologists, according to the classification system. Expressed prostatic secretion （EPS） was collected right before TURP for IL-8 detection using ELISA kits. The resected prostatic tissue was harvested for immunohistochemistry to measure the expression of Bcl-2 and bFGF. Results A total of 30 （60%） patients were confirmed to have prostatic histologic inflammation. The volume of the prostate in BPH with CP was obviously larger than that in simple BPH （P = 0. 022）. The NIH chronic prostatitis symptom index （NIH-CPSI） and international prostate symptom score （IPSS） of BPH with CP were both higher than those of simple BPH （P 〈 0. 05）. Significantly increased levels of IL-8 were observed in EPS obtained from BPH patients with CP （ mean level, 43.29 rig/L）, compared with patients with simple BPH （mean level, 36.90 ng/L） （P =0. 003 ）. The expression of Bcl-2 in BPH with CP （mean level, 6. 17） increased significantly, compared with those in simple BPH （mean level, 2. 45 ） （ P = 0. 013 ）. The expression of bFGF in BPH with CP （ mean level, 7. 57 ） also obviously elevated,compared with those in simple BPH （ mean level, 5.05 ） （ P = 0. 008 ）. Correlation coefficients of IL-8 levels in EPS and expression of Bcl-2 or bFGF indicated that IL-8 had strong correlation with Bcl-2 and bFGF respectively both in simple BPH and BPH with CP （0 〈 R 〈 1, P 〈 0. 05 ）. Conclusions IL-8, Bcl- 2 and bFGF are significantly up

关 键 词：前列腺增生 免疫组织化学 白细胞介素8 碱性成纤维细胞生长因子 Bcl-2 

分 类 号：R697.3[医药卫生—泌尿科学]