自体脂肪基质血管成分对高血压大鼠阴茎勃起功能障碍的影响  

Effects of autologous adipose-derived stromal vascular fraction on erectile dysfunction of hypertensive rats

作　　者：高飞[1] 张胜利[1] 廖胜[1] 葛余正[1] 周六化[1] 吴然[1] 徐郑[1] 许露伟[1] 贾瑞鹏[1] 

机构地区：[1]南京医科大学附属南京医院(南京市第一医院)泌尿外科,210006

出　　处：《中华医学杂志》2016年第2期134-137,共4页National Medical Journal of China

基　　金：国家自然科学基金（81370853、81570613）;江苏省卫生厅科教兴卫工程领军人才与创新团队科研课题（LJ201107）;江苏省六大人才高峰资助项目（2011-WS-093）

摘　　要：目的探讨自体脂肪基质血管成分（SVF）阴茎海绵体注射对大鼠高血压性勃起功能障碍（ED）的影响及其可能机制。方法选择30周龄健康雄性自发性高血压大鼠（SHR）40只，同系正常血压大鼠（WKY）20只，采用无创血压仪测量大鼠尾部收缩压，颈部皮下注射阿扑吗啡（APO）检验大鼠阴茎勃起功能。将有ED的SHR大鼠分为2组：ED—SHR—SVF组（自体SVF阴茎海绵体注射治疗的高血压性ED大鼠，8只）和ED—SHR-磷酸盐缓冲液（PBS）组（PBS阴茎海绵体注射治疗的高血压性ED大鼠，8只）。测定各组大鼠阴茎海绵体内压（ICP），采用Western印迹和RT-PCR法检测阴茎海绵体组织内皮型一氧化氮合酶（eNOS）蛋白和mRNA表达。结果SHR大鼠尾部收缩压高于WKY大鼠[（197．47±6．82）比（125．23±4．65）mmHg，P〈0．05]。SHR大鼠阴茎勃起率为60％（24／40），WKY大鼠为100％（20／20）。5V电刺激后ED-SHR—SVF组ICP明显高于ED—SHR—PBS组[（83．42±3．21）比（52．37±3．11）mmHg，P〈0．05]；ED—SHR—SVF组eNOS蛋白和mRNA表达明显高于ED—SHR—PBS组（0．43±0．03比0．18±0．05、0．92±0．05比0．41±0．06，均P〈0．05）。结论高血压可导致大鼠ED，自体SVF可改善高血压性ED大鼠阴茎勃起功能，其机制可能与大鼠阴茎海绵体eNOS表达上调相关。Objective To study the effect of autologous adipose stromal vascular fraction （SVF） injection into corpora cavernosa on the hypertension-associated erectile dysfunction （ ED ） in rats and its possible mechanism. Methods Healthy male spontaneously hypertensive rats （SHR） at 30-week （n = 40） and homologous rats with normal blood pressure （WKY） （n = 20 ） were selected. Noninvasive blood pressure meter was used to measure the systolic blood pressure （SBP） at the tail. Cervical subcutaneous injection of apomorphine was applied to test penile erectile function. The rats with ED were divided into hypertension-associated ED rats treated with autologous SVF injection into corpora cavernosa （ED-SHR-SVF group） （n = 8 ） and hypertension-associated ED rats treated with phosphate buffered saline （PBS） injection into corpora cavernosa （ ED-SHR-PBS group） （ n = 8）. The intracavernosal pressure （ICP） was measured in each group. Western blot and RT-PCR were conducted to test protein and mRNA expressions of endothelial nitric oxide synthase （ eNOS ） in corpora eavernosa. Results The tail SBP in SHR rats was significantly higher than that in WKY rats （ （ 197.47 ± 6. 82） mmHg vs （ 125.23 ±4. 65 ） mmHg, P 〈 0. 05）. The erectile rate in SHR rats was 60% （24/40）, and that in WKY rats was 100% （20/20）. After 5 V electrical stimulation, the ICP in the ED-SHR-SVF group was significantly higher than in the ED-SHR- PBS group （ （ 83.42 ±3.21 ） mmHg vs （52. 37 ± 3.11 ） mmHg, P 〈 0. 05 ） . The protein and mRNA expressions of eNOS in the ED-SHR-SVF group were significantly higher than in the ED-SHR-PBS group （0.43 ±0.03 vs0.18±0.05, 0.92 ±0.05 vs 0.41 ±0.06, both P〈0.05）.Conclusions High blood pressure can cause ED in rats, which could be mitigated by autologous SVF injection. The mechanism may be related to up-regulation of eNOS expression in corpus cavernosa.

关 键 词：高血压 勃起功能障碍 阴茎海绵体 基质血管成分 

分 类 号：R698.1[医药卫生—泌尿科学]