TTF-1、hMSH-2在先天性肺气道畸形中的表达及意义  

Expression of TTF-1 and h MSH-2 in conqenital pulmonary airway malformation and their implication

作　　者：王利群[1] 周玲玲[2] 周伶俐[2] 王卉芳[1] 王亚楼[1] 钱薇[1] 赵志光[2] 王宗敏[2] 

机构地区：[1]西安市第四医院病理科,西安710004 [2]温州医科大学附属育婴儿童医院病理科,浙江温州325027

出　　处：《诊断病理学杂志》2016年第1期43-46,共4页Chinese Journal of Diagnostic Pathology

基　　金：卫生部国家临床重点专科(温州医科大学附属育英儿童医院儿科呼吸)资助项目

摘　　要：目的探讨甲状腺转录因子-1(TTF-1)和错配修复基因2(h MSH-2)在儿童先天性肺气道畸形(CPAM)中的表达及意义。方法采用免疫组化Eli Vision法检测TTF-1和h MSH-2蛋白在52例先天性肺气道畸形手术存档蜡块标本和9例正常肺组织中的表达。结果在正常儿童肺组织中TTF-1主要在远端细支气管的非纤毛柱状上皮及肺泡Ⅱ型上皮的细胞核内表达。在病例组中,TTF-1主要表达在囊壁的柱状上皮、立方上皮细胞的细胞核内。在CPAMⅠ型、Ⅱ型、Ⅲ型中TTF-1阳性率分别为2%、40%、80%。统计学分析结果表明三者之间表达有明显差异(P<0.01)。h MSH-2蛋白阳性产物在儿童正常肺组织定位于支气管及肺泡上皮细胞的细胞核内,在CPAM中,h MSH-2蛋白染色主要位于囊壁上皮的细胞核内,其阳性率明显低于正常肺组织(P<0.05)。在CPAMⅠ、Ⅱ型的表达无明显差异(P>0.05),但在CPAMⅠ、Ⅱ型中的表达明显强于CPAMⅢ型(P<0.05)。结论 TTF-1在CPAMⅠ、Ⅱ、Ⅲ型中的表达有差异,可以作为CPAM组织分型的一个重要标记。h MSH-2蛋白在病例组CPAMⅠ、Ⅱ、Ⅲ型中表达量均低于正常儿童肺组织,提示在CPAM中有h MSH-2基因转录水平的下降,参与CPAM的形成。Objective To elucidate the role of TTF-1 and hMSH-2 in pathotyping and pathogenesy of congenital pulmonary airway malformation （CPAM） in children through their expression in lesional tissues. Methods Lung tissues of all patients （ ≤ 15-year-old） were recruited in the Affiliated Children Hospital of Wenzhou Medical College and Children Hospital of Zhejiang during 2000 - 2008, including 52 cases of CPAM, 9 cases of pediatric normal lungs. All tissues were diagnosed and grouped through HE after operation. Immunohistochemical staining （EliVision） was used to detect the proteins of TTF-1 and hMSH-2 in every tissue of CPAM and normal lungs. Results Nuclear staining for TTF-1 was observed in nonciliated cells of the bronchial and bronchiolar epithelia and in ceils lining the distal air spaces in normal lungs. In case group, TTF-1 was not detected in the nuclei of epithelial cells lining the cysts in CPAM I . In contrast, TTF-1 was expressed in a majority of the bronchiolar-like epithelial cells of the cysts in CPAM m, where almost 80% of the ceils were TTF-1 positive, and only 40% of the lining cells were TTF-1 positive in CPAM 11 cysts. The difference among CPAM 1 , CPAM I1 and CPAM m was statistically significant （P 〈 0. 05 ）. In groups normal and CPAM cases, immunostaining for hMSH-2 was mainly detected in nuclei of pulmonary epithelial ceils. The expression degree of hMSH-2 in CPAM was significantly weaker than that in normal lungs（ P 〈 0.05 ）. There was no significant difference between CPAM Ⅰ and CPAM Ⅱ, but the difference between CPAM Ⅰ and CPAM Ⅲ was statistically significant （ P 〈 0.05 ） , the difference also existed between CPAM Ⅱ and CPAM m （ P 〈 0. 05 ）. Conclusion The expression of TTF-1 is defferent among CPAM Ⅰ , Ⅱ , Ⅲ. Thus the marker can be used as an important index for different types of CPAM. The expression degree of hMSH-2 in CPAM is significantly weaker than that in normal lungs. Mutation of hMSH-2 could have involved in the formation of CPAM, a

关 键 词：TTF-1 hMSH-2 CPAM 免疫组化 

分 类 号：R562[医药卫生—呼吸系统]