小檗皮水浸膏对db/db糖尿病小鼠视网膜病变的影响(Ⅰ)  

Effects of Water Extract from Cortex of Berberi dictyophylla on Diabetic Retinopathy in Spontaneous Type 2 Diabetic db/db Mice(Ⅰ)

作　　者：叶凡[1] 岳丽珺 范刚[1] 孟宪丽[1] 赖先荣[1] 

机构地区：[1]成都中医药大学,成都611137

出　　处：《中国实验方剂学杂志》2016年第2期82-86,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173360;81303310;81473427;81560806);四川省科技厅科技支撑计划项目(2011SZ0298);四川省教育厅重点项目(10ZA092);四川省高校科研创新团队建设计划项目(11TD004)

摘　　要：目的:观察小檗皮浸膏对基因突变自然发病型db/db糖尿病小鼠视网膜病变的影响。方法:选用18周龄db/db糖尿病小鼠,db/m型同性同窝瘦型小鼠为对照。采用随机方法将db/db小鼠分为:db/db组,小檗皮高、中、低剂量(1.50,0.75,0.38 g·kg^(-1))组,羟苯磺酸钙(0.23 g·kg^(-1))组,盐酸小檗碱(0.135 g·kg^(-1))组。相关药物连续ig 2个月,观察一般体征状态,每2周称体重1次,测空腹血糖水平1次,并于ig 2个月末处死小鼠。苏木素和伊红(HE)染色观察视网膜常规病理改变,消化铺片观察微血管变化,计算视网膜血管内皮细胞/周细胞比值(E/P)。结果:与db/m组比较,db/db组体重显著增加(P<0.01),血糖水平显著升高(P<0.01),视网膜血管E/P明显升高(P<0.05),视网膜毛细血管充血扩张,视网膜损伤明显。与db/db组比较,羟苯磺酸钙组、小檗碱组、小檗皮各剂量组体重无显著变化;小檗碱组、小檗皮低剂量组血糖水平显著降低(P<0.01),小檗皮高、中剂量组血糖水平明显降低(P<0.05),羟苯磺酸钙组血糖水平无显著变化;小檗皮高剂量组视网膜血管E/P显著降低(P<0.05),其他给药组E/P无显著变化;小檗皮各剂量组视网膜损伤减轻。结论:小檗皮对db/db糖尿病小鼠视网膜病变具有明显的防治作用,具有降低自发性糖尿病小鼠高血糖水平作用,改善视网膜微血管损害并阻止新生血管生成。Objective： To observe the effect of water extruct from cortex of Berberi dictyophylla（BDCE）on diabetic retinopathy（DR） in spontaneous type 2 diabetic db / db mice. Method： Eighteen-week-old db / db mice were selected,with their thin db / m mice as normal controls. The db / db mice were randomly divided into 6treatment groups： model control（db / db） group,high and middle and low dosage groups of BDCE（1. 50,0. 75,0. 38 g·kg^-1）,calcium-dobesilate group（0. 23 g·kg^-1）,berberine hydrochloride group（0. 135 g·kg^-1）.During the two-month intragastric administration,all of the mice were weighed and measured for their fasting blood glucose level every two weeks,all the mice were put to death and their eyeballs were taken out at the end of the two-month treatment. Conventional hematoxylin and eosin（HE） staining was performed to observe pathologic change,retina stretched preparation was performed to observe microvascular changes and calculated endothelial /pericytescells（E / P） value. Result： Compared to db / m group,db / db group was significantly higher（P〈0.01）in body mass,fasting blood glucose level（P〈0.01） and E / P value（P〈0.05） of retina microvascular,and showed capillary congestion,expansion and damage of retina. Compared to db / db group,all treatment groups were not significantly higher in body mass; berberine hydrochloride group and low dosage of BDCE group were significantly lower in fasting blood glucose level（P〈0.01）,high and middle dosage group of BDCE were significantly lower in blood glucose level（P〈0.05）,calcium-dobesilate group showed no significant change in fasting blood glucose level; significantly lower in E / P value（P〈0.05） was obtained in high BDCE group,while all of other treatment groups showed no significant change in E / P value,and retinal damage were relieved in all BDCE-treated groups. Conclusion： Spontaneously diabetic db / db mice can be considered as an animal model for human DR. BDCE shows good preventive eff

关 键 词：小檗皮浸膏 基因突变自然发病型db/db糖尿病小鼠 糖尿病视网膜病变 空腹血糖水平 视网膜形态学 

分 类 号：R285.5[医药卫生—中药学]