芪白平肺颗粒对慢性阻塞性肺疾病动物模型的疗效观察  

Observation of Curative Effect of Qibai Pingfei Granules on Rats with Chronic Obstructive Pulmonary Disease

作　　者：常秀娟[1] 孙晓萍[1] 周军[1] 李威[1] 李雪峰[1] 王振中[1] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中国实验方剂学杂志》2016年第2期87-90,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2013ZX09402203)

摘　　要：目的:考察芪白平肺颗粒(QBPF)对脂多糖(LPS)合烟熏致大鼠慢性阻塞性肺疾病(COPD)的治疗作用及其机制研究。方法:正常组于第1,14天气道内注入生理盐水,4周后检测;COPD组于实验第1,14天经气道内注入脂多糖(LPS)200μg/只,香烟烟雾暴露2 h·d^(-1),共4周,观察受试药QBPF剂量(4.4,2.2,1.1 g·kg^(-1))组和氨茶碱(0.054 g·kg^(-1))组灌胃给药后对肺功能和血清白细胞介素^(-1)β(IL-1),肿瘤坏死因子-α(TNF-α),白细胞介素-6(IL-6),干扰素-γ(IFN-γ)含量及肺组织形态学的影响。结果:与正常组比较,模型组最大肺活量(FVC),0.3 s呼气量(FEV 0.3),0.3 s呼气量/最大肺活量(FEV0.3/FVC),呼吸峰流速(PEF)显著下降(P<0.01);血清IL-1β,IL-6,IFN-γ,TNF-a含量明显升高,IL-10水平降低(P<0.01);与模型组比较,芪白平肺颗粒高、中剂量组与氨茶碱组FVC,FEV 0.3,FEV 0.3/FVC,PEF较模型组有明显改善(P<0.05,P<0.01);芪白平肺颗粒血清IL-1β和IL-6,IFN-γ,TNF-a含量明显降低,IL-10水平升高(P<0.05,P<0.01)。结论:QBPF可改善肺功能,减轻肺组织病理变化,减少炎性因子,这可能是治疗慢性阻塞性肺疾病的重要机制。Objective： To investigate the effect of Qibai Pingfei（QBPF） capsule on chronic obstructive pulmonary disease（COPD） rats induced by lipopolysaccharides（LPS） and cigarette smoke,in order to determine its mechanism. Method： Normal rats of control group were bred for 4 weeks in normal circumstance before test and instilled intratracheally with normal saline once at day 1 and day 14. Rats of COPD group were exposed to cigarette smoke 2 h·d^-1 for 4 weeks and intratracheally instilled with 200 μg LPS once at day 1 and day 14. All COPD rats were divided randomly into five groups. Each group was orally administrated with QBPF granules 4. 4,2. 2,1. 1 g·kg^-1 and aminophylline 0. 054 g ·kg^-1 respectively,while model group was administrated with solvent. After 4 weeks,lung function was recorded after administration,level of serum interleukin-1（IL-1β）,tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6）,interferon-γ（IFN-γ） and lung tissue morphology were observed. Result： Compared with normal group,lung function indexes such as forced vital capacity（FVC）,forced expiratory volume in 0. 3 second（FEV 0. 3）,FEV 0. 3 / FVC,peak expiratory flow（PEF） decreased significantly（P〈0.01）; serum levels of IL-1β,and IL-6,IFN-γ,TNF-α significantly increased（P〈0.01）.Compared with model group,lung function indexes such as FVC,FEV 0. 3,FEV 0. 3 / FVC,PEF rose significantly in the middle and high dose of QBPF group and aminophylline group（P〈0.05,P〈0.01）. Compared with model group,QBPF granules group showed significant decrease in Serum levels of IL-1β,and IL-6,IFN-γ,TNF-α but increasein level of IL-10（P〈0.05,P〈0.01）. Conclusion： QBPF granules can improve lung function,reduce the pathological changes of lung tissue. Its inflammatory reduction factors may be an important mechanism for treatment of chronic obstructive pulmonary disease.

关 键 词：芪白平肺颗粒 慢性阻塞性肺疾病 白细胞介素-1Β 肿瘤坏死因子-Α 白细胞介素-6 白细胞介素-10 干扰素-Γ 

分 类 号：R285.5[医药卫生—中药学]