桂枝茯苓胶囊中主要成分对人子宫肌瘤细胞增殖及小鼠离体子宫收缩活动的影响  

Effect of Main Components from Guizhi Fuling Capsule on Human Leiomyoma Cell Proliferation and Contraction of Isolated Mouse Uterine

作　　者：陶晓倩[1] 李娜[1] 曹亮[1] 章晨峰[1] 王团结[1] 丁岗[1] 王振中[1] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中国实验方剂学杂志》2016年第2期91-96,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项项目(2013ZX09402203)

摘　　要：目的:初步评价桂枝茯苓胶囊中17个主要成分抗子宫肌瘤细胞增殖及抗痛经作用。方法:人子宫肌瘤细胞以4×103个/孔接种于96孔板中,加入终质量浓度为25 mg·L^(-1)的17个主要成分作用48 h,每组设6个复孔,采用Hoechst33342/碘化丙啶(PI)双染分析考察17个成分对人子宫肌瘤细胞的体外抑制作用;采用缩宫素(PGF2α)诱导的小鼠离体子宫收缩模型评价17个主要成分的抗痛经生物效应,每组实验重复10次。结果:与空白组比较,槲皮素,桂皮醛,丹皮酚,茯苓酸,去氢土莫酸,白芍苷,芍药苷7个成分能明显抑制子宫肌瘤细胞的增殖,差异均有统计学意义(P<0.05,P<0.01);与空白组比较,没食子酸,芍药苷,丹皮酚,苦杏仁苷,苯甲酰芍药苷,五没食子酰葡萄糖6个成分对小鼠离体子宫平滑肌收缩有明显抑制作用(P<0.01)。结论:槲皮素,桂皮醛,丹皮酚,茯苓酸,去氢土莫酸,白芍苷,芍药苷7个成分可能是桂枝茯苓胶囊治疗子宫肌瘤的主要活性成分;没食子酸,芍药苷,丹皮酚,苦杏仁苷,苯甲酰芍药苷,1,2,3,4,6-O-五没食子酰葡萄糖6个成分可能是桂枝茯苓胶囊治疗原发性痛经的主要活性成分。Objective： To preliminarily evaluate the effect of 17 main components from Guizhi Fuling capsule on human leiomyoma cell proliferation and dysmenorrhea. Method： Human leiomyome cells were grown in 96-well plates and treated with 17 main components（25 mg·L^-1） for 48 h. There were 6 complex wells for each group. Then Hoechst 33342/Propidium iodide（PI） double staining method was used to analyze the effect of the 17 main components on human leiomyome cell,and the anti-dysmenorrhea bioactivity of the 17 components was evaluated by the model Prostag landin F2α（PGF2α）-induced contraction of isolated mouse uterine,with 10 parallel tests for each group. Result： Compared with control group,quercetin,cinnamyl aldehyde,paeonol,pachymic acid,dehydrotumulosic acid,albiflorin and paeoniflorin inhibited human leiomyoma cell proliferation,with significant difference（P〈0. 05,P〈0. 01）. Compared with control group,gallic acid,paeoniflorin,paeonol,laetrile,benzoylpaeoniflorin and 1,2,3,4,6-O-pentagalloylglucose significantly inhibited contraction of isolated mouse uterine（P〈0. 01）. Conclusion： Quercetin,cinnamyl aldehyde,paeonol,pachymic acid,dehydrotumulosic acid,albiflorin and paeoniflorin in Guizhi Fuling capsule may be active ingredients for treating hysteromyoma,while gallic acid,paeoniflorin,paeonol,laetrile,benzoylpaeoniflorin and 1,2,3,4,6-Opentagalloylglucose in Guizhi Fuling capsule may be active ingredients for treating primary dysmenorrhea.

关 键 词：桂枝茯苓胶囊 子宫肌瘤 细胞增殖 痛经 子宫收缩 

分 类 号：R285.5[医药卫生—中药学]