果王素对非小细胞肺癌细胞化疗效果的影响  

Effect of Kiwi essence on Response of Chemotherapy for Non-small Cell Lung Cancer in Mice

作　　者：肖华[1] 邓湘[1] 张平[1] 贺兼斌[2] 

机构地区：[1]南华大学附属第一医院,湖南衡阳421001 [2]怀化市第一人民医院,湖南怀化418000

出　　处：《中国实验方剂学杂志》2016年第2期132-136,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2012年度湖南省卫生厅科研计划项目(2012-81)

摘　　要：目的:观察果王素对肺腺癌小鼠异体移植瘤对化疗药物增敏作用,并初步探讨其作用机制。方法:构建肺腺癌小鼠移植瘤模型,将40只接种Lewis肺癌细胞的C57BL/6J小鼠随机分成5组,每组8只:正常组(生理盐水),果王素组(果王素180 mg·kg^(-1)),化疗组(顺铂联合吉西他滨),60 mg·kg^(-1)果王素联合化疗组,180 mg·kg^(-1)果王素联合化疗组;肿瘤细胞接种后4 d成瘤后给予相应药物干预,接种后20 d处死小鼠,剥离皮下肿瘤。应用Western blot法和RT-PCR检测化疗耐药相关基因切除修复交叉互补基因(ERCC1)和核糖核苷酸还原酶M1(RRM1)的表达。结果:正常组比较,各药物干预组肿瘤的生长明显受到抑制(P<0.01,P<0.05),各组抑瘤率分别为14.95%,41.77%,44.21%,59.68%。正常组ERCC1基因表达最高,各果王素药物干预组表达与正常组比较ERCC1表达明显减少,差异均有统计学意义(P<0.01),果王素组与高剂量果王素联合化疗组表达与其他各组比较明显低于其他组(P<0.01),ERCC1 mRNA表达与ERCC1蛋白表达呈一致性;正常组RRM1基因表达最高,各果王素药物干预组表达与正常组比较RRM1表达明显减少,差异均有统计学意义(P<0.01,P<0.05),果王素组与高剂量果王素联合化疗组表达与其他各组比较明显低于其他组。结论:果王素可以通过降低ERCC1表达及提高RRM1的表达增强化疗药物抑制肿瘤生长及转移的能力。Objective： To investigate the role of Kiwi essence in sensibilization of chemotherapeutics for allotransplantation tumors in mice with lung adenocarcinoma,and preliminarily discuss the related mechanism thereof. Method： Transplantation tumor models were established in mice with lung adenocarcinoma. 40 C57 BL/6J mice bearing Lewis cells were randomized into five groups,n = 8 in each group： normal group（normal saline）,K. essence group（K. essence 180 mg·kg^-1）,chemotherapy group（cisplatin and gemcitabine）,60 mg·kg^-1K.essence combined with chemotherapy group,180 mg·kg^-1K. essence combined with chemotherapy group. After inoculated with adenocarcinoma cells for 4 days,corresponding drugs were given for intervention. Mice were sacrificed 20 days after tumor inoculation,and the subcutaneous tumors were stripped for histological examination.The expressions of excision repair cross-complementation groupl（ERCC1） and ribonucleotidereductase subunit M1（RRM1） for chemotherapy-related drug resistance genes were detected by Western blot assay and RT-PCR.Result： Compared with the normal group,the tumor growth of various drug intervention groups was inhibited significantly（P〈 0. 05, P〈 0.01）,and the anti-tumor rate was 14. 95%,41. 77%,44. 21%,59. 68%respectively in various groups. Expression of ERCC1 gene was highest in normal group,and significantly decreased in various K. essence essence groups,with statistically significant difference（P〈 0.01）. Expression of ERCC1 in K. essence groups and K. essence high dose ＋ chemotherapy group was significantly lower than other groups（P〈 0.01） .ERCC1 mRNA expression and ERCC1 proteins expression were in consistency. Expression of RRM1 gene was highest in normal group,nd significantly decreased in various K. essence groups,with statistically significant difference（P〈 0.01, P〈 0. 05）. Expression of RRM1 in K. essence groups and K. essence high dose ＋chemotherapy group was significantly lower than other groups. Conclusion： K. es

关 键 词：肺肿瘤 腺癌 切除修复交叉互补基因 核糖核苷酸还原酶M1 果王素 

分 类 号：R285.5[医药卫生—中药学]