肺积方对IDO诱导Lewis肺癌小鼠模型免疫逃逸的影响  

Inhibitory Effect of Feiji Recipe on IDO Induced Immune Escape on the Murine Model of Lewis Lung Carcinoma

作　　者：毕凌[1] 金莎[2] 郑展[3] 王青[3] 焦钥 游捷[3] 李和根[3] 田建辉[3] 

机构地区：[1]上海中医药大学研究生院,上海201203 [2]江西省九江学院附属医院西院血液肿瘤科,江西332001 [3]上海中医药大学附属龙华医院肿瘤科,上海市中医药研究院中医肿瘤研究所,上海200032 [4]陕西中医药大学研究生院,陕西712000

出　　处：《中国中西医结合杂志》2016年第1期69-74,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81173224);上海市科学技术委员会科研计划项目(No.11ZR1437000);上海中医药大学附属龙华医院国家中医临床研究基地“龙医团队、龙医学者”建设计划项目(No.D-11);国家中医临床研究基地业务建设科研专项课题(No.JDZX2012125)

摘　　要：目的研究肺积方对吲哚胺-2,3-双加氧酶(indoleamine 2,3-dioxygenase,IDO)诱导Lewis肺癌(Lewis lung carcinoma,LL/2)小鼠模型免疫逃逸的影响。方法采用C57BL/6小鼠建立LL/2-增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)-IDO模型48只,将小鼠按随机数字表分为模型组(灌胃生理盐水)、中药组[灌胃肺积方水煎液100mg/(g·d)]、1-甲基-D-色氨酸(1-methyl-D-tryptophan,1-MT)治疗组[灌胃1-MT混合液100mg/(kg·d)]和紫杉醇组[腹腔注射紫杉醇15mg/(kg·d)],每组12只。造模后第2日开始干预,其中24只观察小鼠生存期,余24只在造模并干预28日后采用流式细胞术检测脾脏CD4^+CD25^+FoxP3^+调节性T细胞(regulatory T cells,Treg)比例。结果与模型组比较,中药组、1-MT组生存期延长(P<0.01),中药组、1-MT组、紫杉醇组Treg比例降低(P<0.01)。与紫杉醇组比较,中药组、1-MT组生存期延长(P<0.01),1-MT组Treg比例降低(P<0.05)。结论肺积方能抑制肺癌细胞的增殖,抑制免疫逃逸,改善免疫功能,延长荷瘤小鼠生存期。Objective To study the effect of Feiji Recipe（FR）intervening indoleamine 2,3-dioxygenase（IDO）induced immune escape on the murine model of Lewis lung carcinoma.Methods Totally 48 C57BL/6 mice inoculated with Lewis lung cancer cells transfected with human（enhanced green fluorescent protein,EGFP）-IDO gene were divided into four groups according to radom digit table,i.e.,the model group（administered with normal saline by gastrogavage）,the Chinese medicine group（treated with FR Decoction at the daily dose of 100 mg/g by gastrogavage）,the 1-methyl-D-trytaphan（1-MT）group（administered with 1-MT mixed liquor at the daily dose of 100 mg/kg by gastrogavage）,and the Paclitaxel group（treated with Paclitaxel at the daily dose of 15 mg/kg by peritoneal injection）,12 in each group.The intervention was started from the 2nd day of modeling.The survival time was observed in24 of them.Ratios of CD4^＋CD25^＋FoxP3^＋regulatory T cells（Treg）in the spleen were detected in the rest 24 mice by flow cytometry respectively.Results Compared with the model group,the survival time was significantly prolonged inthe Chinese medicine group and the 1-MT group（P〈0.01）;ratios of Treg cells remarkably decreased in the Chinese medicine group,the 1-MT group,and the Paclitaxel group（P〈0.01）.Compared with the Paclitaxel group,the survival time was significantly prolonged in the Chinese medicine group and the 1-MT group（P〈0.01）;ratios of Treg cells decreased significantly in the 1-MT group（P〈0.05）.Conclusion FR could inhibit the proliferation of lung cancer cells and immune eseape,improve the immune function,and prolong the survival of tumor-bearing mice.

关 键 词：肺肿瘤 肺积方 1-甲基-D-色氨酸 吲哚胺2 3-双加氧酶 微环境 免疫逃逸 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]