拜颤停复方对帕金森病小鼠模型多巴胺的影响  

Effect of Baichanting Compound on Dopamine Contents in Parkinson′s Disease Model Mice

作　　者：任燕冬[1,2] 井月娥[1] 张淑香[1] 王洪玉[1] 卢芳[1] 刘树民[1] 

机构地区：[1]黑龙江中医药大学药物安全性评价中心,哈尔滨150040 [2]黑龙江中医药大学佳木斯学院,黑龙江154007

出　　处：《中国中西医结合杂志》2016年第1期94-98,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金青年科学基金资助项目(No.81303249)

摘　　要：目的观察拜颤停复方组分配比对帕金森病(Parkinson′s disease,PD)小鼠纹状体内多巴胺(dopamine,DA)的影响,筛选最优配比。方法以MPTP诱导C57BL/6小鼠建立PD模型,采用均匀设计的实验方法,将组成拜颤停复方的3味中药提取物按不同配比(刺五加提取物X1∶白芍提取物X2∶钩藤提取物X3=30.00∶34.92∶82.50,48.00∶19.98∶72.19,18.00∶44.88∶61.88,36.00∶29.94∶51.56,54.00∶15.00∶41.25,24.00∶39.90∶30.94,42.00∶24.96∶20.63)进行干预,模型组和正常组灌胃等量5%羧甲基纤维素钠,连续干预20日。运用超高效液相色谱-串联质谱(ultra performance liquid chromatography tandem mass spectrometry,UPLC-MS)方法,观察各组小鼠DA含量。除正常组10只外,筛选PD模型小鼠20只,随机数字表法分为模型组和拜颤停复方组(优化配比组方),拜颤停复方组灌胃优化配比提取物,模型组和正常组灌胃等量0.5%羧甲基纤维素钠,连续干预20日。观察各组小鼠行为学,检测纹状体内DA含量。结果与正常组比较,模型组小鼠纹状体内DA含量降低(P<0.01),提示造模成功。与模型组比较,1、2组纹状体内DA含量升高(P<0.05),根据二次多项式逐步回归统计结果,得出回归方程为Y=0.265+0.026X2-0.056X3+0.334×10^(-3)×X1×X3+0.691×10^(-3)×X3~2,钩藤提取物为影响药效的主因素(P<0.01),由回归方程预测其最佳配伍为:X1(刺五加提取物)=54.00mg/(kg·d),X2(白芍提取物)=44.88mg/(kg·d),X3(钩藤提取物)=82.50mg/(kg·d),拜颤停复方组爬杆时间缩短,自主活动次数升高,DA含量升高,差异均有统计学意义(P<0.01,P<0.05)。结论拜颤停复方能增加帕金森病小鼠纹状体DA的含量,最优配比为54.00∶44.88∶82.50。Objective To observe the effect of Baichanting Compound（BC）on dopamine（DA）in striatum of Parkinson′s disease（PD）mice,and to screen the optimal component proportion.Methods The PD model was established in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）induced C57BL/6 mice.By using uniform design,they were intervened by three extracts of BC in different proportions[Acanthopanax senticosus extract（X1）∶white peony root extract（X2）∶Uncaria rhynchophyllaextract（X3）=30.00∶34.92∶82.50,48.00∶19.98∶72.19,18.00∶44.88∶61.88,36.00∶29.94∶51.56,54.00∶15.00∶41.25,24.00∶39.90∶30.94,42.00∶24.96∶20.63）.Equal volume of 5% carboxymethylcellulose sodium was administered to mice in the model group and the normal group by gastrogavage.All medication was lasted for 20 successive days.The dopamine（DA）content was determined by ultra performance liquid chromatography tandem mass spectrometry（UPLCMS）.Except 10 in the normal group,20 PD model mice were screened and divided into the model group and the BC group（with the optimal proportion）according to random digit table.BC extract in optimal proportion was administered to mice inthe BC group by gastrogavage,while equal volume of 5%carboxymethylcellulose sodium was administered to mice in the model group and the normal group by gastrogavage.All medication was lasted for 20 successive days.Praxiology was observed in each group.DA content in striatum was also detected.Results Compared with the normal group,the DA content in striatum decreased significantly in the model group（P〈0.01）,suggesting a successful PD modeling.Compared with the model group,the DA content in striatum increased significantly in1 and2 groups（P〈0.05）.According to results of quadratic polynomial stepwise regression statistics,the regression equation obtained was：Y=0.265＋0.026X2-0.056X3＋0.334×10^-3×X1×X3＋0.691×10^-3×X3^2.X3 extract was the main factor influencing the effectiveness（P〈0.01）.The optimal proportion of BC wa

关 键 词：拜颤停复方 帕金森病 多巴胺 均匀设计 

分 类 号：R285.5[医药卫生—中药学]