柱前衍生化-HPLC法测定大蒜中甾体皂苷的含量  

Content Determination of Garlic Saponin in Allium sativum by Pre-column Derivatization-HPLC

作　　者：宋兴良[1] 梁恕坤[1] 赵翊萌 王鹏[1] 

机构地区：[1]临沂大学化学化工学院,山东临沂276005

出　　处：《中国药房》2016年第3期361-364,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.21275068);临沂市科学技术发展计划项目(No.2010GSF10222)

摘　　要：目的：建立测定大蒜中甾体皂苷含量的方法。方法：以对硝基苯甲酰氯为衍生化剂对大蒜中甾体皂苷进行柱前衍生化,采用高效液相色谱法测定其含量。色谱柱为Shimadzu VP-ODS,流动相为乙腈-水（80∶20,V/V）,流速为1.0 ml/min,检测波长为254 nm,柱温为25℃,进样量为20μl。结果：菝葜皂苷检测质量浓度线性范围为0-1.25 mg/ml（r=0.9990）;精密度、稳定性、重复性试验的RSD〈2%;加样回收率为93.1%-96.8%（RSD=1.56%,n=6）。结论：该方法操作简便、稳定、重复性好,可用于大蒜中甾体皂苷含量的测定。OBJECTIVE： To establish a method for content determination of steroidal saponin in Allium sativum. METHODS： Pre-column derivatization of steroidal saponin was performed by using the derivatization agent of nitro-benzoic acid-chlorine. And HPLC was conducted to determine the content of steroidal saponin. The column was Shimadzu VP-ODS with mobile phase of acetonitrile - water mixed solution （80：20, V/V） at a flow rate of 1.0 ml/min, the detection wavelength was 254 nm, the column temperature was 25℃, and the injection volume of 20μl. RESULTS： The linear rang of sarsasapogenin was 0-1.25 mg/ml（r=0.999 0）; RSDs of precision, stability and reproducibility tests were lower than 2%; recovery was 93.1%-96.8% （RSD=1.56%, n= 6）. CONCLUSIONS： The method is simple, stable with good separation, and can be use for the content determination of steroidal saponin in A. sativum.

关 键 词：大蒜 柱前衍生化 高效液相色谱法 甾体皂苷 菝葜皂苷 

分 类 号：R927.2[医药卫生—药学]