HPLC法测定旋覆花药材中旋覆花素和去乙酰旋覆花素的含量  

Simultaneous Determination of Inulicin and Deacetylinulicin in Inulae Flos by HPLC

作　　者：马立满 刘震[1] 尚明英[1] 刘广学[1] 徐风[1] 蔡少青[1] 

机构地区：[1]北京大学药学院生药学研究室,北京100191

出　　处：《中国药房》2016年第3期369-371,共3页China Pharmacy

基　　金：国家药品标准提高暨2015年版<中国药典>科研任务项目

摘　　要：目的：建立测定旋覆花药材中旋覆花素和去乙酰旋覆花素含量的方法。方法：采用高效液相色谱法。色谱柱为Zorbax SB-C18,流动相为乙腈-水（梯度洗脱）,流速为1.0 ml/min,柱温为25℃,检测波长为210 nm,进样量为10μl。结果：旋覆花素和去乙酰旋覆花素的检测质量浓度线性范围分别为0.0002-0.005、0.0001-0.0017μg/ml（r=0.9998、0.9994）;精密度、稳定性、重复性试验的RSD〈2%;加样回收率分别为99.63%-103.56%、95.98%-101.21%,RSD分别为1.26%、1.84%（n=9）。结论：该方法操作简便,结果准确、可靠,可用于评价旋覆花药材的质量。OBJECTIVE： To establish a method of simultaneous determination of inulicin and deacetylinulicin in Inulae Flos. METHODS： HPLC was performed on the column of Zorbax SB-C18 with mobile phase of acetonitrile-water（gradient elution）at a flow rate of 1.0 ml/min, the column temperature was 25 ℃, the detection wavelength was 210 nm, and the injection volume was 10 μl. RESULTS： The linear range was 0.000 2-0.005 μg/ml（r=0.999 8） for inulicin and 0.000 1-0.001 7 μg/ml （r=0.999 4） for deacetylinulicin; RSDs of precision, stability and reproducibility tests were lower than 2.0% ; recoveries were 99.63%-103.56% （RSD= 1.26%, n=9） and 95.98 %-101.21% （RSD= 1.84%, n=9）, respectively. CONCLUSIONS： The method is simple, accurate and reliable, and can be used for the quality evaluation of Inulae Flos.

关 键 词：旋覆花 旋覆花素 去乙酰旋覆花素 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]