离子色谱法测定骨瓜提取物注射液中糖的含量  

Content Determination of Carbohydrates in Osseous and Cucumis Extract Injection by Ion Chromatography

作　　者：张伟[1] 张彤[1] 任连杰[1] 吕雯[1] 韩春霞 周长明[1] 

机构地区：[1]北京市药品检验所,北京100035 [2]赛默飞世尔科技(中国)有限公司,北京100007

出　　处：《中国药房》2016年第3期386-389,共4页China Pharmacy

摘　　要：目的：建立测定骨瓜提取物注射液中糖含量的方法。方法：采用离子色谱法。色谱柱为Dionex Carbo Pac PA20,流动相为水-1 mol/L醋酸钠溶液-250 mmol/L氢氧化钠溶液（梯度洗脱）,流速为0.45 ml/min,柱温为35℃,进样量为10μl,检测器为电化学分析检测器。结果：半乳糖、葡萄糖、甘露糖、蔗糖、果糖检测质量浓度线性范围均为0.5-10.0μg/ml（r〉0.9980）;精密度、稳定性、重复性试验的RSD〈2%;各组分加样回收率为94.8%-99.1%,RSD=0.52%-0.89%（n=9）。结论：该方法简单、快速、准确,可用于骨瓜提取物注射液中糖的测定。OBJECTIVE： To establish a method for the content determination of carbohydrates in Osseous and cucumis extract injection. METHODS： Ion chromatography was conducted on the column of Dionex CarboPac PA20 with mobile phase of water-1 mol/L Sodium acetate solution-250 mmol/L Sodium hydroxide solution （gradient elution） at flow rate of 0.45 ml/min, column temperature was 35 ℃, and injection volume was 10μl, detector was electrochemical analysis detector. RESULTS： The linear ranges of galactose, glucose, mannose, sucrose and fructose were 0.5-10.0 μg/ml（r〉0.998 0）; RSDs of precision, stability and reproducibility tests were lower than 2 % ; recovery was 94.8 %-99.1% （RSD = 0.52 %-0.89 %, n = 9）. CONCLUSIONS ： The method is simple, fast and accurate, and can be used for the determination of carbohydrates in Osseous and cucumis extract injection.

关 键 词：离子色谱法 骨瓜提取物注射液 糖 

分 类 号：R927.2[医药卫生—药学]