HPLC法同时测定儿泻停糖浆中黄芩苷、盐酸小檗碱、葛根素的含量  

Simultaneous Determination of Puerarin,Berberine Hydrochloride and Baicalin in Erxieting Syrup by HPLC

作　　者：周越[1] 陈建伟[2] 

机构地区：[1]南京中医药大学附属连云港市中医院药学部,江苏连云港222000 [2]南京中医药大学药学院,南京210046

出　　处：《中国药房》2016年第3期394-396,共3页China Pharmacy

摘　　要：目的：建立同时测定儿泻停糖浆中葛根素、盐酸小檗碱、黄芩苷含量的方法。方法：采用高效液相色谱法。色谱柱为Kromasil C18,流动相为甲醇-0.2%磷酸水溶液（梯度洗脱）,流速为1.0 ml/min,柱温为30℃,检测波长为275 nm,进样量为10μl。结果：黄芩苷、盐酸小檗碱、葛根素的检测质量浓度线性范围分别为10.43-83.44、10.71-85.68、12.58-100.64μg/ml;精密度、稳定性、重复性试验的RSD〈2%;加样回收率分别为98.25%-100.87%、99.41%-101.40%、98.13%-99.76%,RSD分别为0.92%、0.68%、0.57%（n=9）。结论：该方法操作简便,结果可靠,可用于儿泻停糖浆的质量控制。OBJECTIVE： To establish a method for simultaneous determination of puerarin, berberine hydrochloride and baicalin in Erxieting syrup. METHODS： HPLC was performed on the column of Kromasil-C18 with mobile phase of methanol-0.2% Phosphoric acid solution （gradient elution） at flow rate of 1.0 ml/min, column temperature was 30 ℃, detection wavelength was 275 nm, and the volume injection was 10 μl. RESULTS： The linear was 10.43-83.44 μg/ml for puerarin, 10.71-85.68 μg/ml for berberine hydrochloride and 12.58-100.64 μg/ml for baicalin （r≥0.999 3）; RSDs of precision, stability and reproduciblity tests were lower than 2%;recoveries were 98.25%-100.87%（RSD=0.92%,n=6）,99.41%-101.40%（RSD=0.68%,n=6） and 98.13%-99.76% （RSD = 0.57 %, n = 6）, respectively. CONCLUSIONS： The method is simple, reliable, can be used for the quality control of Erxieting syrup.

关 键 词：儿泻停糖浆 黄芩苷 盐酸小檗碱 葛根素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]