SSR标记辅助回交选育抗草甘膦油菜新品系的研究  

Development of New Transgenic Glyphosate-Resistant Rapeseed Lines Assisted by SSR Marker Selection

作　　者：张荣[1,2] 信晓阳[1,2] 胡胜武[1,2] 

机构地区：[1]旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]西北农林科技大学农学院,陕西杨凌712100

出　　处：《西北农业学报》2016年第1期64-71,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：现代农业产业技术体系建设专项资金(CARS-13);"十一五"国家科技支撑计划(2010BAD01B02)~~

摘　　要：为快速培育抗除草剂草甘膦的油菜新品系,以含草甘膦氧化还原酶基因gox的转基因抗草甘膦材料C9为供体亲本,以优良甘蓝型冬油菜Gr-1、Gr-2为轮回亲本,以回交转育为基本方法,利用gox基因的PCR检测和喷除草剂2种方法对回交后代进行前景选择,并利用SSR分子标记进行背景辅助选择,将抗草甘膦基因gox导入Gr-1、Gr-2中,在BC3选育得到遗传背景回复率大于96%的抗草甘膦油菜新品系。By using Canadian rapeseed（Brassica napus L.）line C9 with glyphosate resistance which conferred by the goxgene as the donor parent,and Gr-1,Gr-2,two elite local rapeseed lines as recurrent parents,glyphosate resistance was successfully transferred into Gr-1and Gr-2backgrounds by combination of detection of goxgene by PCR（Polymerase Chain Reaction）method and the spraying glyphosate solution method,and SSR（simple sequence repeats）marker assistant background selection.In BC3,glyphosate resistant plants with〉96% genetic background recovery rates were successfully developed.

关 键 词：油菜 草甘膦抗性 回交转育 SSR标记 辅助选择 

分 类 号：S565.4[农业科学—作物学]