茶树‘紫娟’肉桂酰辅酶A还原酶基因(CCR)克隆及序列分析  

Cloning and Sequence Analysis of Cinnamoyl-CoA Reductase Gene from Tea Plant[Camellia sinensis(L.)O.Kuntz]

作　　者：陈林波[1] 宋维希[1] 李晓霞[1] 夏丽飞[1] 周萌[1] 梁名志[1] 焉文光 

机构地区：[1]云南省农业科学院茶叶研究所云南省茶树种质资源创新与配套栽培技术工程研究中心,云南勐海666201

出　　处：《西北农业学报》2016年第1期80-85,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31560220;31460216);云南省农业科学院专项基金(YAAS2013JC001;YAAS2014JC012);茶树生物学与资源利用国家重点实验室开放基金(LTBB20140103)~~

摘　　要：通过克隆茶树肉桂酰辅酶A还原酶基因(CCR)的cDNA序列,为研究其蛋白结构和功能奠定基础。对利用cDNA-AFLP技术获得的‘紫娟’茶树成熟叶片上调的差异表达片段TDF,设计特异引物,利用RACE末端扩增技术,分别扩增出5′端和3′端目的片段,测序后进行拼接获得茶树肉桂酰辅酶A还原酶基因(CCR)cDNA全长序列,并进行序列分析。结果表明:克隆的茶树CsCCR基因cDNA全长1 259bp(GenBank登录号为KJ995737),其中开放阅读框957bp。同源比对发现,与蓖麻、丹参、草莓、番茄的CCR蛋白同源性分别为67%、68%、69%和70%。In order to lay a foundation for the structure and function studies of CsCCRprotein,CsCCR cDNA of tea plant was cloned.The cDNA-AFLP was applied to up-regulated transcript derived fragment（TDF）from mature leaves of tea plant（Camellia sinensis var.assamica,cultivar ‘Zijuan＇）.TDF was used to design specific primers,and then cloning the 5′-and 3′-end sequence by RACE,and fragment was assembled to get completely cDNA.Finally,CsCCRcDNA sequence was analyzed.The results show that the cDNA of CsCCR was 1 259 bp in length（GenBank accession No.KJ995737）,and contains an open reading frame（ORF）of 957 bp and encoding 319 amino acids residues.It was found that the amino acid sequence of CsCCRhad 67%,68%,69% and 70% homology with Ricinus communis,Salvia miltiorrhiza,Fragaria vesca,and Solanum lycopersicum,respectivly.

关 键 词：茶树 肉桂酰辅酶A还原酶 基因克隆 序列分析 

分 类 号：S571.1[农业科学—茶叶生产加工]