南海短尾大眼鲷线粒体Cytb基因序列及种群判别分析  

Sequence analysis of the mitochondrial cytochrome b gene and identification of the Priacanthus macracanthus population in the South China Sea

作　　者：熊丹[1,2] 李敏[1] 李永振 李玉芳[1,2] 张魁[1] 陈作志[1] 

机构地区：[1]中国水产科学研究院南海水产研究所、农业部南海渔业资源开发利用重点实验室、农业部南海渔业资源环境科学观测实验站,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306 [3]农业部南海区渔政局,广东广州510080

出　　处：《中国水产科学》2016年第1期188-197,共10页Journal of Fishery Sciences of China

基　　金：农业部财政重大专项(NFZX2013);国家科技支撑计划项目(2013BAD13B06);中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2014TS23)

摘　　要：对采集于中国南海北部近岸和南沙西南部陆架海域7个采样点共246尾短尾大眼鲷（Priacanthus macracan-thus）样品进行了线粒体细胞色素b基因（cytochromeb，cyt6）序列的扩增与分析，获得了长度为684bp的同源序列。碱基A、T、C、G含量分别为22．7％、28．4％、33．4％、15．5％，共检测到多态位点92个，定义了90个单倍型。其遗传多样性表现出高单倍型多样性（0．8130～0．9012）和低核苷酸多样性（0．0040～0．0053）的特点。两两群体间的Fs。分析显示，大部分群体间的只。值均小于0．05，且差异不显著（p〉0．05），样本总体分化指数仅为0．012，群体间分化程度很低。分子方差分析（AMOVA）表明，群体间的遗传变异仅占总遗传变异的1．25％，98．75％的遗传变异源于群体内，群体之间有较高的遗传同质性。基于邻接法构建的系统发育树和基于中间连接法构建的单倍型网络图均显示，南海短尾大眼鲷不存在与地理群体对应的支系，遗传分化不显著。中性检测和不对称分布分析发现，南海短尾大眼鲷群体在更新世晚期发生过种群扩张。结果表明南海短尾大眼鲷群体间的遗传交流较频繁，可以认为是一个大的种群。Understanding the population structure of fish species could provide the basic information for utilization and management of fishery. The Red bigeye Priacanthus macracanthus was one of the economically important fish species that was widely distributed in the South China Sea. However, little was known about the populations. In the present study, the population genetic structure and diversity ofP. macracanthus were examined in the South China Sea based on a 684 bp segment of the mitochondrial cytochrome b gene. A total of 264 individual samples were collected from seven localities in the northern South China Sea and the Southwestern Nansha Islands. The bases A, T, C, and G had frequen- cies of 22.7%, 28.4%, 33.4%, and 28.4%, and A ＋ T content was similar to that ofC ＋ G （51.1% and 48.9%, respec- tively）. Ninety-two polymorphic loci were detected and 90 haplotypes were defined. Genetic diversity was high for haploid type （0.8130-0.9012） but nucleotide diversity was low （0.0040-0.0053）. A haploid type network diagram found advantages for the Hapl, Hap2, Hap4, and Hap6 haploid types, but no significant geographical features were detected. A pairwise Fat analysis showed that most of the/：st value were low and non-significant （P〉0.05）. The overall Fst for the total sample was only 0.012, which showed little differentiation among populaitons. Analysis of molecular variance （AMOVA） indicated that most of the differentiation occurred within populations （98.75%） and only 1.25% occurred among populations. Phylogenetic tree constructed using neighbor-joining method showed that the seven groups of P macracanthus were a panmictic population, with no obvious type branches. Haplotype network using median-joining network approach also revealed no significant genealogical clades corresponding to sampling localities. Both neutrality tests and mismatch distribution analyses indicated a recent population expansion in P. macracanthus during the last gla- cial maximum. These results indicate high gene fl

关 键 词：种群判别 短尾大眼鲷 细胞色素B 南海 

分 类 号：S931[农业科学—渔业资源]