大花旋蒴苣苔对脱水与复水的生理响应  

Physiological response of Boea clarkeana to dehydration and rehydration

作　　者：张丹丹[1] 周守标[1,2] 周会[1] 刘芳[1] 杨世勇[1] 马志慧[1] 

机构地区：[1]安徽师范大学生命科学院,安徽芜湖241000 [2]安徽师范大学环境科学与工程学院,安徽芜湖241000

出　　处：《生态学杂志》2016年第1期72-78,共7页Chinese Journal of Ecology

基　　金：安徽省自然科学基金项目(11040606M77);安徽省高校自然科学基金重点项目(KJ2011A129);安徽省高校生物环境和生态安全重点实验室专项基金(2004sys003)资助

摘　　要：本试验以陕西、四川(下称西部)、安徽和浙江(下称东部)4省区大花旋蒴苣苔(Boea clarkeana Hemsl.)的离体叶片为材料,通过对不同脱水时间(0、4、8、12、24和48 h)和脱水48 h后不同复水时间(4、8、12、24和48 h)条件下大花旋蒴苣苔生理指标变化的研究,比较了中国东部和西部地区大花旋蒴苣苔的耐脱水特性。结果表明:4种群大花旋蒴苣苔的相对含水量在脱水过程中呈显著下降趋势(P<0.05);相对含水量下降的总体趋势表现为浙江种群>安徽种群>四川种群>陕西种群;在复水过程中,4种群相对含水量基本恢复到实验前水平;超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)3种抗氧化酶活性以及MDA、脯氨酸、可溶性糖和可溶性蛋白的含量在脱水过程中均表呈显著上升趋势(P<0.05),在复水过程中均呈显著下降趋势(P<0.05)。通过对4种群生理指标的比较研究发现,4种群的耐脱水性整体表现为陕西种群>四川种群>安徽种群>浙江种群,即西部种群的耐旱性强于东部地区。In order to investigate the dehydration tolerance and physiological responses of Boca clarkeana, detached leaves of four populations of B. clarkeana from Shanxi, Sichuan （ hereafter west China）, Anhui and Zhejiang （hereafter east China） provinces were subjected to dehydration for 0, 4, 8, 12, 24 and 48 h, respectively, and 48 h after dehydration the same leaves were subjected to rehydration again for 4, 8, 12, 24 and 48 h, respectively. The results showed that the relative water contents of the four populations decreased significantly during the entire dehy- dration process （P〈0.05）. The general dehydration tolerance of the four populations was in order of Zhejiang population 〈 Anhui population 〈 Sichuan population 〈 Shanxi population. The rela- tive water contents in the four populations could be recovered to their original states during rehy- dration. The activities of antioxidant enzymes （ SOD, POD, CAT） and contents of osmoregulation substances （MDA, proline, soluble protein, soluble sugar） increased significantly during desic- cation and decreased significantly during rehydration （P〈0.05）. In comparison, the dehydration tolerance of the four populations of B. clarkeana were Shanxi population 〉 Sichuan population 〉 Anhui population 〉 Zhejiang population. The west populations of B. clarkeana had higher dehy- dration tolerance than the east populations.

关 键 词：复苏植物 大花旋蒴苣苔 脱水与复水 生理响应 抗氧化酶 渗透调节物质 

分 类 号：Q945[生物学—植物学]