氮、磷、铁对三角褐指藻诱变株生长、总脂及脂肪酸的影响  

Effects of nitrogen,phosphorus and iron on the growth,total lipid content and fatty acid composition of Phaeodactylum tricornutum mutant strain

作　　者：梁晶晶[1] 蒋霞敏[1] 叶丽[1] 韩庆喜[1] 

机构地区：[1]宁波大学海洋学院,浙江宁波315211

出　　处：《生态学杂志》2016年第1期189-198,共10页Chinese Journal of Ecology

基　　金：国家海洋公益性项目(201305022);浙江省近岸水域与保护重点试验室开放基金项目(J2012010)资助

摘　　要：为了优化微藻培养条件,采用单因子试验研究了不同氮浓度(5、10、15、20、25、30和35 mg·L^(-1))、不同磷浓度(0.5、1.0、1.5、2.0、2.5、3.0和3.5 mg·L^(-1))、不同铁源(Fe Cl3、Fe C6H5O7和Fe SO4)和铁浓度(0、0.10、0.25、0.50和1.00 mg·L^(-1))对三角褐指藻紫外诱变株MP-2的影响。结果表明:氮、磷、铁对MP-2的生长、总脂含量和脂肪酸组成影响显著(P<0.05);MP-2生长最适氮浓度为20 mg·L^(-1),其生长速率K值和生物量分别为(0.384±0.004)和(0.25±0.01)g·L^(-1),氮浓度为30 mg·L^(-1)时总脂积累量最高(26.51±1.96)%,氮浓度25 mg·L^(-1)时PUFA积累最高[(37.78±0.35)%];MP-2生长最适磷浓度为2.5 mg·L^(-1),生长速率K值和生物量分别为(0.305±0.010)和(0.28±0.02)g·L^(-1),磷浓度1.0 mg·L^(-1)时总脂积累量最高[(21.79±0.89)%],磷浓度为2.5 mg·L^(-1)时PUFA积累最高[(39.33±0.38)%];Fe SO4为铁源时生长最佳,显著优于其余各组(P<0.05),生长最适铁浓度为0.50 mg·L^(-1),生长速率K值和生物量分别为(0.495±0.006)和(0.87±0.04)g·L^(-1),铁浓度0.25 mg·L^(-1)时总脂积累量最高[(28.86±0.50)%],铁浓度1.0mg·L^(-1)时PUFA积累最高[(41.73±0.42)%]。由此可见,MP-2生长、总脂和PUFA积累所需的氮、磷、铁需求量不同,在生产性培养时最好采用二次培养。To optimize the cultivation conditions of microalgae, single-factor test was performed to reveal the effects of different nutritional conditions on Phaeodactylum tricornutum mutant strain MP-2, including nitrogen （5, 10, 15, 20, 25, 30, 35 mg ·L-1） , phosphorus （0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 rag- L-l）, iron sources （FeC13, FeC6HsOT, FeSO4） and their concentra- tions （0, 0.10, 0.25, 0.50, 1.00 mg · L-1）. The results showed that all these factors exerted significant effects on the growth, total lipid content and fatty acid composition of MP-2 （P 〈0.05）. The highest growth rate was obtained with the nitrogen concentration of 20 mg · L-1, with the growth rate K values and biomass of 0.384±0.004 and （0.25±0.01） g · L-1, respective- ly. The highest total lipid content （26.51± 1.96） % was achieved when the nitrogen concentration was 30 mg · L-1, and the highest PUFA content （37.78±0.35） % occurred when it was 25 mg · L-1. MP-2 got the fastest growth and highest lipid content when the phosphorus concentra- tion was 2.5 mg· L-1, and the growth rate K value and biomass were 0.305±0.010 and （0.28± 0.02） g · L-1, respectively. The total lipid content achieved the highest value （21.79±0.89） % when the phosphorus concentration was 1.0 mg · L-1, and the highest PUFA content occurred when it was 2.5 mg · L-1. cant difference from other FeSO4 was proved to be iron sources （ P 〈 0. 05 ） the optimal iron source, and showed signifi The optimal FeSO4 concentration was 0.50mg · L-1, with growth rate K value and biomass of （0.495±0.006） and （0.87±0.04） g· L-1, re- spectively. The highest total lipid content （28.86±0.50） % was achieved when the FeSO4 concen- tration was 0.25 mg · L-1, and the highest PUFA content occurred when it was 1.0 mg · L-1. The highest growth rate and total lipid and PUFA contents were obtained with different concentrations of nitrogen, phosphorus and iron, so a cultivation strategy should be adopted in mass culture.

关 键 词：氮 磷 铁 三角褐指藻诱变株 生长 总脂 脂肪酸 

分 类 号：Q949.2[生物学—植物学]