薯蓣皂苷元体外对大鼠滑膜RSC-364细胞株STAT3及VEGF影响的研究  

Effects of Diosgenin on STAT3 and VEGF expression in rat synovial cell-364 in vitro

作　　者：郭亚春[1] 高亚贤[2] 宋鸿儒[2] 梁秀军[2] 冯建 

机构地区：[1]承德医学院病原生物学教研室,河北承德067000 [2]承德医学院免疫学教研室,河北承德067000 [3]承德市双桥区双峰寺中心卫生院,河北承德067000

出　　处：《现代预防医学》2016年第1期128-130,150,共4页Modern Preventive Medicine

基　　金：国家自然科学基金资助项目(No.30873420)

摘　　要：目的通过IL-17联合TNF-α刺激大鼠成纤维细胞样细胞株(Rat Synovial Cell-364,RSC-364)建立类风湿性关节炎(Rheumatoid Arthritis,RA)滑膜炎性细胞模型,观察薯蓣皂苷元含药血清对RA滑膜炎性细胞模型酪氨酸蛋白激酶家族-信号转导及转录激活子转导途径中信号转导及转录激活子3(Signal Transducer and Activator of Transcription 3,STAT3)和血管内皮生长因子(Vascular Endouthelial Growth Factors,VEGF)表达水平的影响,初步探讨薯蓣皂苷元抗RA滑膜炎病变的作用机制。方法 RSC-364细胞建立滑膜炎性细胞模型后,分模型组(B组)、雷公藤片组(C组)、薯蓣皂苷元组(D组),另设正常对照组(A组)。各组STAT3蛋白通过Western blot方法检测,VEGFm RNA表达采用实时定量聚合酶链式反应(Polymerase Chain Reaction,PCR)检测。结果 STAT3蛋白、VEGFm RNA表达在B组显著高于A组(P<0.05);经薯蓣皂苷元及雷公藤片含药血清作用后,STAT3蛋白、VEGFm RNA表达明显降低(P<0.05)。药物组间STAT3蛋白、VEGFm RNA表达无明显区别(P>0.05)。结论薯蓣皂苷元可能通过抑制STAT3蛋白,进而抑制VEGF表达,通过抑制血管新生,减轻RA关节炎症反应。Objective To observe the effects of medicated serum with Diosgenin on STAT3 and VEGFm RNA expression in rat synovial cell strain RSC-364 induced by IL-17 and TNF-α. To investigate the underlying mechanism of Diosgenin in treating rheumatoid arthritis（RA）. Methods Rat synovial cell strain RSC-364 were stimulated by IL-17＋ TNF-α to established RA cell model. Experiment was divided into 4 groups（n=6）： normal control group, model group, diosgenin and tripterygium group. The STAT3 and VEGFm RNA expression in rat synovial cell strain RSC-364 in each group was detected by Western blot and real-time quantitative PCR. Results Compared with the control group, the STAT3 and VEGFm RNA expression in model group increased remarkably（P〈0.05）. The STAT3 and VEGFm RNA expression in tripterygium group and diosgenin group were remarkably lower than the model group（P〈0.05）. Conclusion Diosgenin might inhibit the expression of STAT3 protein in the JAK-STAT signal transduction pathway to inhibit the expression of VEGFm RNA, thereby to restrain angiogenesis of RA and relieve arthritis response.

关 键 词：薯蓣皂苷元 类风湿性关节炎 血管新生 STAT3 VEGF 

分 类 号：R181.24[医药卫生—流行病学]