NaCl胁迫下两种春小麦新品种渗透性调节反应的比较  

Comparison osmotic adjustment in two new spring wheat varieties under NaCl stress

作　　者：杨颖丽[1] 李翠祥[1] 滕玉瑾 马婷[1] 许富强[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2016年第1期93-99,共7页Journal of Northwest Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(31360094;31470464)

摘　　要：以春小麦新品种陇春30和陇春27为材料,研究了不同浓度NaCl(25,100和200mmol·L-1)处理对其渗透性调节物含量及脯氨酸代谢相关酶活性的影响.结果显示,盐胁迫诱导两种小麦叶片中可溶性糖含量及叶、根中脯氨酸含量升高,可溶性蛋白含量只在200 mmol·L-1 NaCl处理的陇春27根中增加为对照的156%.与对照相比,25和100mmol·L-1 NaCl处理导致陇春30叶片中鸟氨酸转氨酶(OAT)活性显著升高,而陇春27叶片中的该酶活性在100和200mmol·L-1盐处理下降低.不同的是,200mmol·L-1 NaCl处理的陇春30根中OAT活性升高,25和200mmol·L-1NaCl处理的陇春27根中该酶活性也升高.此外,盐胁迫下两种小麦幼苗叶和根中谷氨酸激酶(GK)和脯氨酸脱氢酶(PDH)活性均减弱.结果表明,盐胁迫诱导两种小麦陇春30和陇春27幼苗叶、根中渗透性调节物含量发生了不同程度的变化,特别是脯氨酸的积累显著,且陇春30叶和陇春27根中脯氨酸的积累主要与乌氨酸(Orn)合成途径及PDH活性减弱有关,而可能与谷氨酸(Glu)合成途径无关.New spring wheat（cv Longchun 30 and Longchun 27）seedlings are used to investigate changes of osmolyte content and proline metabolism enzyme activities in response to different NaCl concentrations（25,100 and 200mmol·L-1）.Soluble sugar content in leaves as well as proline levels in leaves and roots rise in two wheat seedlings under salinity tress.The amount of soluble protein increases to 156% of control value in 200mmol·L-1 NaCl-treated roots of Longchun 27 seedlings.Compared with the control,ornithine aminotransferase（OAT）activity rises in response to 25 and 100mmol·L-1 NaCl in Longchun 30 leaves and decreases about 14% and 39% to 100 and 200 mmol·L-1 NaCl in Longchun 27 leaves,respectively.Differently,200 mmol·L-1 NaCl-treated Longchun 30 roots exhibite increased OAT activity,and 25 and 200mmol·L-1 NaCl also stimulate this enzyme in Longchun 27 roots.In addition,the activities of glutamate kinase（GK）and proline dehydrogenase（PDH）lower in leaves and roots when two wheat seedlings are exposed to different NaCl concentrations.Taken together,salt stress induces different degrees of changes in osmolyte content in leaves and roots of two wheat seedlings,especially proline accumulation is significant. Here it is suggested that proline accumulation in the leaves of Longchun 30 and the roots of Longchun 27 may be associated with ornithine（Orn）synthesis pathway and the decrease of PDH activity,but not due to glutamic（Glu）synthesis pathway in the leaves and roots of two wheat seedlings under salinity stress.

关 键 词：NACL胁迫 渗透性调节物 脯氨酸代谢 小麦 

分 类 号：S512.12[农业科学—作物学]