姜黄素类似物L6H4对2型糖尿病大鼠心肌的保护作用  

Effects of curcumin analogue L6H4 on myocardial tissue of type 2 diabe tic rats

作　　者：应丽丽[1] 倪瑾瑶 李慧敏[1] 余霞[1] 陈霖[1] 陈三妹[2] 陈国荣[1] 

机构地区：[1]温州医科大学附属第一医院病理科,浙江温州325015 [2]绍兴文理学院医学院,浙江绍兴312000

出　　处：《中国病理生理杂志》2016年第1期27-32,共6页Chinese Journal of Pathophysiology

基　　金：浙江省公益性技术应用研究计划(No.2011C23123);温州市高层次人才创新技术项目

摘　　要：目的:探讨姜黄素类似物L6H4对2型糖尿病心肌病大鼠的保护作用及机制。方法:雄性SD大鼠40只,随机均分为正常对照(NC)组、高脂(HF)组、高脂治疗(FT)组、糖尿病(DM)组和糖尿病治疗(DT)组。采用高脂饮食加链脲佐菌素诱导2型糖尿病模型,用L6H4治疗8周。生化法测血糖及血脂水平,ELISA法测血清脂联素(APN)水平,放射免疫法测大鼠血清胰岛素水平,并计算胰岛素抵抗指数(HOMA-IR),Masson染色和电镜观察心肌形态改变,免疫组化测心肌脂联素受体1(AdipoR1)及转化生长因子β1(TGF-β1)蛋白的表达。Western blot法检测大鼠心肌内AdipoR1蛋白表达量。结果:HF组及DM组大鼠血糖、血脂、胰岛素、HOMA-IR及TGF-β1蛋白水平较NC组均有升高,L6H4治疗后均下降;HF组及DM组大鼠血清APN水平及心肌AdipoR1较NC组均有下降,L6H4治疗后均升高。DM组和HF组大鼠心肌纤维化,线粒体肿胀,嵴紊乱,部分消失,经L6H4干预后,大鼠心肌的形态学损害明显减轻。结论:L6H4对2型糖尿病大鼠的心肌具有保护作用;血清APN浓度增加,心肌AdipoR1蛋白表达增强,及APN抑制TGF-β1的表达可能参与其中。AIM： To explore the effects of curcumin analogue L6H4 on the myocardial tissue of type 2 diabetic rats and its mechanism. METHODS： Male Sprague-Dawley rats were randomly divided into normal control（ NC） group,high-fat（ HF） group,high-fat treatment（ FT） group,diabetes mellitus（ DM） group and diabetes treatment（ DT） group.The rats in the latter 4 groups were fed high-fat diet for 4 weeks,then the rats in DM groups and DT groups were intraperitoneally injected with streptozotocin（ STZ） to induce type 2 diabetes,while the rats in FT group and DT group were given L6H4. The blood glucose and lipid levels were detected by biochemical method,and serum adiponectin（ APN） levels were detected by ELISA. The serum insulin levels were measured by radioimmunoassay and homeostasis model assessment of insulin resistance（ HOMA-IR） were calculated. The morphological changes of myocardium were observed by Masson staining and electron microscopy. The protein expression of adiponectin receptor 1（ AdipoR1） and transforming growth factor β1（ TGF-β1） in myocardial tissue were determined by immunohistochemistry. The protein expression of adipoR1 was also detected by Western blot for verification. RESULTS： Compared with NC group,the blood glucose,lipids,insulin,HOMAIR and TGF-β1 were increased in HF and DM group,but they were decreased after treated with L6H4. Compared with NC group,the concentration of serum APN were decreased and the expression of AdipoR1 in the myocardium were weakened in HF group and DM group,and they increased after treated with L6H4. The myocardial fibrosis was obvious in HF group and DM group,the mitochondria in cardiomyocytes expanded,and the cristae disordered,partial disappeared. These lesions were significantly reduced after L6H4 treatment. CONCLUSION： L6H4 exerts a protective effect on the heart in type 2diabetic rats. The increased concentration of serum APN,the enhanced expression of AdipoR1,and the expression of TGF-β1 inhibited by APN may be involved in th

关 键 词：糖尿病 心肌 姜黄素类似物L6H4 脂联素 脂联素受体1 转化生长因子Β1 

分 类 号：R587.1[医药卫生—内分泌] R363[医药卫生—内科学]