新型多吡啶锰(Ⅱ)配合物的合成、晶体结构与DNA反应性及抗肿瘤活性  

Synthesis,crystal structure,interaction with DNA and antiproliferative activity of cancer cells of polypyridyl Mn(Ⅱ) complex

作　　者：黄冉涛 李政道[1] 赵强[1] 陈文涛[1] 杨浩[1] 

机构地区：[1]南阳师范学院化学与制药工程学院,河南南阳473061

出　　处：《化学研究与应用》2016年第1期19-24,共6页Chemical Research and Application

基　　金：河南省科技厅基础与前沿技术研究项目(No.112300410189)资助项目

摘　　要：合成了一种新型多吡啶锰(Ⅱ)配合物,用元素分析、红外光谱、X-射线单晶衍射等手段对配合物结构进行了表征。该配合物晶体属单斜晶系,P2(1)/c空间群。用电子吸收光谱、荧光光谱和凝胶电泳法研究了配合物与DNA的相互作用,同时采用MTT法研究了该配合物对人乳腺癌细胞(MCF-7)和人肺癌细胞(A549)的体外生长增殖抑制活性。结果表明,配合物与CT DNA作用属部分插入和静电结合模式,其键合常数K_b=3.3×10~4L·mol^(-1)。凝胶电泳实验研究表明配合物用310 nm光辐射15 min,可将质粒p BR322 DNA切割为开环缺口型和线型DNA。体外抗肿瘤活性实验发现,合成配合物能有效抑制MCF-7和A549肿瘤细胞增殖。A new polypyridyl Mn（ Ⅱ） complex has been synthesized and characterized by elemental analysis,IR and X-ray single crystal diffraction.The crystal structure of complex belongs to monoclinic system with space group P2（ 1）/c.Interactions of the complex with DNA binding have been investigated by absorption spectroscopy,fluorescence spectroscopy and agarose gel electrophoresis.The antiproliferative activity of the complex was investigated against human breast carcinoma（ MCF-7） and human lung carcinoma（ A549） by MTT assay.The spectrophotometric studies showed that the mode of binding could be partial intercalation and electrostatic interaction and its binding constant Kbis 3. 3 ×10~4L·mol-1. The agarose gel electrophoresis showed complex can cleave circular plasmid p BR322 DNA to both nicked and linear forms when it was irradiated at 310 nm for 15 min.. The in vitro anticancer activity assay of the complex indicates that the complex can effectively inhibit the proliferation.

关 键 词：多吡啶配体 锰(Ⅱ)配合物 晶体结构 DNA 光裂解 抗肿瘤 

分 类 号：O614[理学—无机化学]