石榴皮中活性成分鞣花酸的平衡溶解度与油水分配系数的测定  

Determination of equilibrium solubility and oil-water partition coefficient of ellagic acid

作　　者：施洋[1,2] 孙芸[2,3] 谢莉[2] 田莉[2,3] 

机构地区：[1]新疆医科大学学报编辑部,乌鲁木齐830011 [2]新疆医科大学中医学院,乌鲁木齐830011 [3]新疆医科大学新疆名医名方与特色方剂学重点实验室,乌鲁木齐830011

出　　处：《新疆医科大学学报》2016年第2期145-148,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(81260668);新疆医科大学“天山英才工程”专项(Y0382002)

摘　　要：目的考察鞣花酸在25℃和37℃下不同p H磷酸盐缓冲液中的平衡溶解度与表观油水分配系数（Papp）。方法采用紫外-可见分光光度法测定鞣花酸在不同p H缓冲液中的平衡溶解度;采用摇瓶法测定鞣花酸的Papp值。结果 25℃时鞣花酸在p H值为1.2、2.5、5.0、5.8、6.8、7.4、7.8～8.0的磷酸盐缓冲液中的平衡溶解度分别为2.77、4.97、8.14、6.16、1.22、1.33、1.01 g/mL,相应条件下Papp值分别为-3.20、-2.36、-2.49、-2.50、-2.48、-2.37、-2.22;37℃时鞣花酸在上述p H缓冲液中的平衡溶解度为3.25、5.08、8.04、6.39、1.02、1.54、1.14g/mL,相应条件下的Papp值分别为-2.38、-2.32、-2.38、-2.41、-2.44、-2.38、-2.18。结论鞣花酸在酸性溶液中的平衡溶解度随p H值的升高而增大,在中性及弱碱性溶液中其溶解度较小。在p H值为1.2～8.0时,鞣花酸的Papp值均〈-2,表明鞣花酸不易透过生物膜,推测其在胃肠道的吸收不好,且温度对鞣花酸的Papp值几乎无影响。Objective To determine the equilibrium solubility and the apperent oli / water partion coefficient（ Papp）of ellagic acid in different p H phosphate buffer solutions in 25℃ and 37℃. Methods Ultraviolet spectrophotometry was employed to determine the equilibrium solubility of ellagic acid in phosphate buffer solutions of different p H value,and the Pappvalues of ellagic acid were determined by shaking flask. Results At 25℃ the equilibrium solubility of ellagic acid in different phosphate buffer solutions of p H 1. 2,2. 5,5. 0,5. 8,6. 8,7. 4,7. 8-8. 0 were 2.77,4. 97,8. 14,6. 16,1. 22,1. 33,1. 01 g / mL,and the Pappvalues of ellagic acid were-3. 20,-2. 36,-2. 49,-2.50,-2. 48,- 2. 37,- 2. 22 respectively; At 37℃ the equilibrium solubility of ellagic acid in different phosphate buffer solutions of p H 1. 2,2. 5,5. 0,5. 8,6. 8,7. 4,7. 8-8. 0 were 3. 25,5. 08,8. 04,6. 39,1. 02,1. 54,1. 14 g /mL,and the Pappvalues of ellagic acid were-2. 38,-2. 32,-2. 38,-2. 41,-2. 44,-2. 38,-2. 18 respectively.Conclusion The equilibrium solubility of ellagic acid is increasing with p H increases in acidic solution and its solubility is less in neutral and weak alkaline solution. The Pappvalues of ellagic acid were -2 at the p H range from 1. 2 to 8. 0,which shows it is not easy to through the biological membrane and the absorption is poor in the gastrointestinal tract. The temperature has little influence on the Papp values of ellagic acid.

关 键 词：鞣花酸 平衡溶解度 表观油水分配系数 

分 类 号：R943[医药卫生—药剂学]