石油工人ET-1基因Lys198Asn位点与职业紧张和高血压关系的研究  

Oil workers ET-1 gene Lys198Asn site relationship between occupational stress and hypertension

作　　者：张馨怡[1] 姜雨[1] 赵云娟[2] 刘继文[1] 

机构地区：[1]新疆医科大学公共卫生学院,乌鲁木齐830011 [2]新疆医科大学基础医学院免疫学教研室,乌鲁木齐830011

出　　处：《新疆医科大学学报》2016年第2期235-238,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(81260424;81260425)

摘　　要：目的探讨石油工人ET-1基因Lys198Asn位点与职业紧张和高血压的关系。方法 (1)采用整群抽样的方法,抽取新疆石油工人1 473名作为研究对象,利用职业紧张量表(OSI-R)职业任务问卷(ORQ)测定其紧张程度,分析1 425份有效问卷;(2)抽取其中337名石油工人,病例组(血压≥140/90 mmHg)155名,对照组182名,采集血样进行DNA提取,并用RT-PCR的方法检测Lys198Asn位点,计数资料采用卡方检验,等级资料采用秩和检验。结果在不同职业紧张水平下,高血压的患病率差异有统计学意义(P<0.05),不同职业紧张水平石油工人是否患高血压的人数差异有统计学意义(P<0.05),高血压病例组和对照组Lys198Asn位点的不同基因型分布差异无统计学意义(P>0.05),不同紧张水平组的高血压与Lys198Asn位点基因型差异无统计学意义(P>0.05)。结论石油工人中,紧张程度高的是输油工。Lys198Asn位点可能与高血压的患病与否无关,不同紧张水平的石油工人患高血压可能与Lys198Asn位点无关。Objective To research the oil workers ET-1 gene Lys198 Asn site relationship between occupational stress and hypertension. Methods A total of 1 473 Xingjiang oil workers were selected by cluster random sampling and measured the level of their occupational stress with the occupational stress inventory revised edition（ OSI-R） and occupational role questionnaire（ ORQ）. 1 425 questionnaires were valid. 337 oil workers were selected,including 155 oil workers as hypertension group（ blood pressure≥140 /90 mmHg） and 182 oil workers as control group. The blood samples were taken of each subject,and extracted DNA to detecte Lys198 Asn gene with R T-PCR to know hypertension susceptibility gene in this population. Chi-square test was used for enumeration data and rank sum test was used for ranked data. Results Under the different levels of occupational stress,the prevalence of hypertension was statistically significant（ P〈0. 05）,different occupational stress level was suffering from the different risk of hypertension,the different was statistically significant（ P〈0. 05）,different genotypes of Lys198 Asn sites were no significant differences in the hypertension group and control group（ P〉0. 05）,Lys198 Asn site genotype in different level of occupational stress with hypertension was no statistical significance（ P〉0. 05）. Conclusion In oil workers,oil transportation workers have higher occupational stress. Lys198 Asn site may be has no relationship with hypertension,Different levels of oil workers suffering from hypertension or not may be unrelated to Lys198 Asn site.

关 键 词：石油工人 职业紧张 Lys198Asn 高血压 

分 类 号：R13[医药卫生—劳动卫生] R34[医药卫生—公共卫生与预防医学]