长枝木霉T6菌株对黄瓜南方根结线虫的防治及其根际定殖作用  

Efficiency of Trichoderma longibrachiatum T6 in the control of Meloidogyne incognita and its rhizosphere colonization in cucumber

作　　者：张树武[1] 徐秉良[1] 薛应钰[1] 梁巧兰[1] 刘佳[1] 

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,兰州730070

出　　处：《应用生态学报》2016年第1期250-254,共5页Chinese Journal of Applied Ecology

基　　金：草业生态系统教育部省部共建重点实验室项目(CY-GG-2006-013);甘肃省农牧厅生物技术专项项目(GNSW-2013-19);甘肃省教育厅项目(042-03)资助~~

摘　　要：采用温室盆栽试验测定了不同浓度长枝木霉T6菌株孢子悬浮液对南方根结线虫的防治及在黄瓜根际的定殖作用.结果表明:根际定殖处理10周后黄瓜根际土壤和根系中长枝木霉T6菌株菌落数量显著增加,且处理土壤和根系中菌落数量变化显著,浓度为1.5×10~7CFU·m L^(-1)处理的根际土壤和根系中菌落数量达到7.8×10~7和6.3×10~5CFU·m L^(-1).温室防治试验中,不同浓度长枝木霉T6菌株孢子悬浮液对南方根结线虫不同虫态具有较强的防治效果,且防治效果随着孢子悬浮液浓度的增加而增加.长枝木霉T6菌株孢子悬浮液对被南方根结线虫侵染的黄瓜株高、根系长度和鲜质量具有显著的促生作用.表明长枝木霉T6菌株在黄瓜根际具有较强的定殖作用,对南方根结线虫具有较好的防治作用,并对黄瓜植株生长具有显著的促生作用.Efficiency of different concentrations of Trichoderma longibrachiatum T6 against Meloidogyne incognita and its rhizosphere colonization in cucumber were determined in greenhouse experiments. The results of rhizosphere colonization experiments showed that the number of colonies in cucumber soil and root increased significantly ten weeks after inoculation with the second stage juveniles of M. incognita and different concentrations of T. longibrachiatum T6,and there was significant difference in different concentrations of T. longibrachiatum T6,e.g.,the maximum numbers of colonies in soil and root were 7.8×10^7 and 6.3×10^5CFU·mL^-1 respectively after treated with the spore suspension of 1.5×10^7CFU·mL^-1. Greenhouse experiments results showed that different concentrations of T. longibrachiatum T6 had significant control effect on different life stages of M. incognita,and the control effect increased with the concentration of T. longibrachiatum T6. T. longibrachiatum T6 significantly increased plant height,root length,above-ground and root fresh mass of cucumber inoculated by M. incognita. T. longibrachiatum T6 could colonize in cucumber rhizosphere,had control effect on M. incognita,and significantly improved the growth of cucumber.

关 键 词：木霉菌 南方根结线虫 防治效果 定殖作用 

分 类 号：S476[农业科学—农业昆虫与害虫防治]