苯并[a]芘与铅对小鼠学习记忆能力影响协同效应  

Synergistic effect of benzo[a]pyrene and lead on learning and memory of mice

作　　者：杨凯[1] 秦启忠[2] 涂白杰[1] 

机构地区：[1]重庆医科大学公共卫生学院环境卫生与劳动卫生教研室,重庆400016 [2]重庆医科大学实验教学中心

出　　处：《中国公共卫生》2016年第1期85-88,共4页Chinese Journal of Public Health

基　　金：国家自然科学基金(81372957)

摘　　要：目的探讨苯并[a]芘(BaP)与铅对小鼠学习记忆能力影响之间的协同效应。方法将80只昆明小鼠随机分为10组:空白对照组,溶剂对照组(等量花生油),低、高铅组(5.4、54.0 mg/L),低、高Ba P组(0.5、5.0mg/kg),低铅+低Ba P组,低铅+高Ba P组,高铅+低Ba P组,高铅+高Ba P组;采用M orris水迷宫评定小鼠空间学习记忆能力,单细胞凝胶电泳检测海马神经元DNA损伤。结果与对照组比较,低、高铅组,低、高Ba P组,低铅+低Ba P组,低铅+高Ba P组,高铅+低Ba P组,高铅+高Ba P组小鼠逃避潜伏期[分别为(25.66±2.93)、(28.43±3.24),(23.56±2.69)、(33.54±3.82),(28.52±3.25),(43.57±4.97),(40.26±4.59),(54.54±6.22)s]明显延长,而跨越平台次数及目标象限花费时间明显减少,差异均有统计学意义(P<0.001);与对照组比较,低、高铅组,低、高Ba P组,低铅+低Ba P组,低铅+高Ba P组,高铅+低Ba P组,高铅+高Ba P组小鼠尾部DNA百分比(分别为0.09%、0.15%,0.12%、0.21%,0.17%,0.31%,0.31%,0.51%)明显增加(F=9.823、5.226,P<0.05)。结论苯并[a]芘与铅联合对小鼠空间学习记忆损伤具有协同效应,其机制可能与氧化应激损伤有关。Objective To investigate the synergistic effect of benzo[a ] pyrene （BaP） and lead on learning and mem- ory of mice. Methods Eighty male Kunming mice were randomly divided into 10 groups：control group, vehicle control （sesame oil） ,low（5.4 mg/L）and high（54 mg/L）dose lead acetate groups,low（0.5 mg/kg bw）and high（5 mg/kg bw）dose BaP groups,low dose lead ＋ low dose BaP group,low dose lead ＋ high dose BaP group,high dose lead ＋ low dose BaP group, and high dose lead ＋ high dose BaP group. The capability of learning and memory of the mice were measured with Morris water maze （MWM）test and the DNA damage of neuron in the hippocampus was assessed with single-cell gel electrophoresis（SCGE）. Results Compared with the control groups,both BaP- and lead-exposed animals showed significant increase in escape latency （ 25.66 ± 2. 93,28. 43 ±3.24,2. 56± 2. 69,33.54± 3.82,28. 52 ± 3.25, 43.57 ±4. 97,40. 26 ±4. 59,and 54. 54 ±6. 22, all P 〈0. 001 ） ;however,the numbers crossing the exact location of the platform and the time spent in the target quadrant were significantly decreased（ all P 〈 0. 001 ）in MWM test. The animals in experimental groups also showed a significant increase in the percentage of DNA in tail （0. 09% ,0. 15% ,0. 12%, 0. 21%, 0. 17%, 0. 31%, 0. 31%, and 0. 51% ） compared with those of the control groups （ 0. 08%, F = 9. 823, P = 0. 1301 ;F = 5. 226 ,P = 0. 012）. Conclusion The combination of BaP and lead exposure could induce spatial learning and memory impairments synergistically in mice and the mechanisms of the synergistic effects may be due to oxidative stress injury.

关 键 词：苯并[a]芘(BaP) 铅 协同效应 学习记忆缺损 海马 

分 类 号：R277.791.6[医药卫生—中医学]