牛膝总皂苷对过氧化氢致人脐静脉内皮细胞损伤的保护作用研究  

Protective Effect of Achyranthes Bdentata Saponins on Human Umbilical Vein Endothelial Cell injured by Hydrogen Peroxide

作　　者：王菊平[1] 吴晓东[1] 宋献美 

机构地区：[1]郑州市儿童医院,河南郑州450003 [2]河南医学高等专科学校,河南郑州451191

出　　处：《亚太传统医药》2016年第2期10-12,共3页Asia-Pacific Traditional Medicine

基　　金：河南省高等学校重点科研项目计划(15B310004)

摘　　要：目的:观察牛膝总皂苷对过氧化氢(H2O2)诱导人脐静脉内皮细胞(HUVEC)损伤的保护作用。方法:体外培养HUVEC,分为空白对照组、模型对照组及牛膝总皂苷低、中、高浓度组,MTT法检测细胞活力,微板法检测上清液中一氧化氮(NO)的含量,采用ELISA法检测内皮素-1(ET-1)、组织型纤溶酶原激活物(tPA)、纤溶酶原激活物抑制剂(PAI-1)的含量。结果:与模型对照组比较,牛膝总皂苷中、高浓度组细胞活力显著升高,ET-1和PAI-1含量显著减少,NO和tPA含量显著升高(P<0.05或P<0.01)。结论:牛膝总皂苷对H2O2诱导的HUVEC损伤具有显著的保护作用。Objective ： To observe the protective effect of Achyranthes bidentata saponins on the injury of human umbilical veinendo- thelial cell （HUVEC） injured by hydrogen peroxide. Methods：The HUVEC were cultured vitro and divided into five groups as fol lows： control group, model group, low-dosage of Achyranthes bidentata saponins group, mid dosage of Achyranthes bidentata sapo- nins capsule group and high-dosage of Achyranthes bidentata saponins capsule group. MTT was used to determine the HUVEC via- bility. The contents of nitric oxide（NO） were measured by the microplate and the method of enzyme-linked immunosorbent assay （ELISA） was used to determin endothelin-1 （ET-1）, tissue plasminogen activator （tPA） and plasminogen activator inhibitor-1 （PAI- l）. Results： Compared with the model group, the mid-dosage of Achyranthes bidentata saponins and high-dosage of Achyranthes bidentata saponins significantly increased HUVECs viability, decreased the contents of ET-1, PAI 1 and increased the contents of NO and tPA（P〈0.05 or P〈0.01）. Conclusion： Achyranthes bidentata saponins has significant protective effect on HUVEC in-

关 键 词：过氧化氢损伤 牛膝总皂苷 人脐静脉内皮细胞 作用机制 

分 类 号：R285[医药卫生—中药学]