乌梅丸对溃疡性结肠炎大鼠结肠上皮细胞凋亡和Bcl-2/Bax蛋白表达的影响  

Study on Effect of Wumei Pill in Colonic Epithelial Cell Apoptosis and Expression of Bcl-2/Bax of Ulcerative Colitis in Rats

作　　者：惠毅[1] 闫曙光[1,2] 王晓龙[1] 

机构地区：[1]陕西中医学院,陕西咸阳712046 [2]陕西省胃肠病证方药省级重点研究室,陕西咸阳712046

出　　处：《中华中医药学刊》2016年第1期149-151,I0014-I0016,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：陕西省中医药管理局项目(13-jc020);陕西省科技厅项目(2013JQ4006)

摘　　要：目的:观察乌梅丸对溃疡性结肠炎大鼠结肠上皮细胞凋亡和Bcl-2/Bax蛋白表达的影响,揭示该方治疗溃疡性结肠炎的作用机制。方法:以SD大鼠为研究对象,采用TNBS/乙醇灌肠诱导UC形成,通过乌梅丸灌胃给药,TUNEL法检测结肠上皮细胞的凋亡,免疫组化法检测结肠上皮Bcl-2、Bax表达。结果:与空白组比较,模型组大鼠结肠上皮细胞凋亡显著增多,Bcl-2蛋白表达减弱,Bax蛋白的表达增强(P<0.05);与模型组比较,乌梅丸大、中、小剂量组大鼠结肠上皮细胞凋亡明显减少,Bcl-2蛋白表达增强,Bax蛋白的表达减弱(P<0.05)。结论:乌梅丸可通过抑制结肠上皮细胞的过度凋亡对溃疡性结肠炎起治疗作用,其作用机制与调节Bcl-2和Bax基因表达密切相关。Objective： To observe the effect of Wumei Pill in colonic epithelial cell apoptosis and expression of Bcl-2 / Bax of ulcerative colitis rats and to explore its effect mechanism on treating ulcerative colitis. Methods： Male SD rats were selected to establish ulcerative colitis model by using an enema of TNBS and ethanol. All the rats except blank group were given Wumei Pill and then we tested the colonic epithelial cell apoptosis in TUNEL method and Bcl- 2 and Bax expressions in immunohistochemical method of rats＇ colonic tissue. Results： Compared with blank group,the colonic epithelial cell apoptosis and the expression of Bax were significantly enhanced while Bcl- 2 was decreased in colonic epithelial cells in model group rats（ P 〈0. 05）. Compared with model group,the colonic epithelial cell apoptosis and the expressions of Bax were significantly decreased while Bcl- 2 was enhanced in colonic epithelial cells in Wumei Pill high,middle and low groups（ P 〈0. 05）. Conclusion： Wumei Pill has therapeutic effect on ulcerative colitis through inhibiting the excessive apoptosis of colon epithelial cells and the mechanism of action has been closely related to regulating the expressions of Bcl- 2 and Bax.

关 键 词：溃疡性结肠炎 乌梅丸 结肠上皮细胞凋亡 BCL-2 BAX 

分 类 号：R285.5[医药卫生—中药学]