气虚血瘀证荷瘤小鼠复合模型的建立及活血化瘀法对其干预的影响研究  

Establishing Mice Model of Colorectal Carcinoma with Qi Deficiency and Blood Stasis Syndrome and Observation on Effects of Promoting Blood Circulation Therapy

作　　者：钱丽燕[1] 郭勇[2] 李妍[3] 唐朋林[4] 

机构地区：[1]浙江中医药大学附属第二医院,浙江杭州310005 [2]浙江中医药大学附属第一医院,浙江杭州310006 [3]浙江中医药大学,浙江杭州310053 [4]浙江中医药大学附属第三医院,浙江杭州310005

出　　处：《中华中医药学刊》2016年第1期177-180,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科学研究基金计划(A类)项目(2012ZA049)

摘　　要：探索气虚血瘀证结肠腺癌移植瘤的小鼠复合模型的建立及中药活血化瘀法干预对其影响。方法:健康8周龄Balb/c雌性小鼠72只随机分成6组,分别为:空白对照组(A组)、单纯荷瘤对照组(B组)、复合模型对照组(C组)、复合模型低剂量组(D组)、复合模型中剂量组(E组)、复合模型高剂量组(F组)。造模第16天起,A、B、C组给予生理盐水灌胃,D、E、F组分别按高、中、低剂量的四君子合血府逐瘀汤灌胃,每天1次连续4周。对造模前后小鼠进行症状和体征的评价,第42天,将已禁食12 h的各组小鼠称重、麻醉,检测局部微循环血流量,眼球取血以送检血液流变学。结果:1各复合模型组小鼠量化评分积分评价气虚血瘀程度皆为重度,但高剂量组活血化瘀药干预后较复合对照组量化积分明显减轻,差异有统计学意义(P<0.05)。2全血黏度按空白对照组、单纯荷瘤组、复合模型组呈梯度递增,运用中药干预后,呈现不同程度的下降。3空白组的局部血流量大于荷瘤组及复合模型组所测得血流值,其中耳缘血管(P<0.01)腹壁静脉(P<0.05)差异最为显著。复合模型组随活血化瘀药剂量的增加局部微循环血流量有逐步增加。4至造模第42天,单纯荷瘤组与复合模型组体质量均减轻,与空白组比较有极显著差异(P<0.01)。复合模型组体质量与单纯荷瘤组相比有减轻,随活血化瘀药剂量增加差异逐渐减小。结论:1本实验建立了气虚血瘀状态下Balb/c小鼠结肠腺癌复合模型,符合中医证型特点。2对气虚血瘀证结肠腺癌移植瘤的小鼠复合模型分别采用低、中、高剂量的活血化瘀中药进行干预,能不同程度减少气虚血瘀的症状与体征。Objective： To establish a mice model of colorectal carcinoma with Qi deficiency and blood stasis syndrome and to observe the effects of promoting blood circulation therapy. Methods： 72 female Balb / c mice,which were 8 weeks old,were randomly divided into 6 groups,blank control group（ group A）,tumor- bearing mice group（ group B）,control group with tumor（ group C）,tumor- bearing mice group with low dose herb medicine（ group D）,tumor- bearing mice group with middle dose herb medicine（ group E）,tumor- bearing mice group with high dose herb medicine（ group F）.From the sixth day on,mice in group D,E and F were poured the corresponding dosages of herb medicine into stomach every day for 4 weeks while the rest groups were poured with NS. We evaluated signs and symptoms in mice. On the 42 th day,the mice fasting for 12 hours were sacrificed and the blood flow in the local area was detected. And then blood taken out by removing eyeball was sent to exam the hemodynamic indexes. Results： 1Mice in group C,D,E and F had a high score in Qi deficiency and blood stasis quantization scale but score of group F was significantly reduced than that of the group C and the difference was obvious（ p〈0. 05）. 2Whole blood viscosity from low to high was group A,B,C. After the intervention of TCM,there was different degrees of decline. 3Regional blood flow of mice in group A was bigger than that in group B,C,D,E and F and the difference between the ear edge of vascular blood flow（ P〈0. 01） and abdominalwall blood flow（ P 〈0. 05） was obvious. Regional blood flow of mice in group D,E and F gradually increased. 4Till the42 th day,the weights of group B,C,D,E and F reduced and the difference was obvious when compared with group A（ P〈0. 01）. Group C,D,E and F had lighter weight than group B and the difference decreased with the drug dose increasing.Conclusion： 1The study set the mice model of colorectal carcinoma with Qi deficiency with blood stasis syndrome in line with the characteristics

关 键 词：疾病模型 结肠癌 气虚血瘀证 小鼠 

分 类 号：R285.5[医药卫生—中药学]