高效液相色谱法测定复方丹参方滴丸中三七总皂苷的含量  

Content Determination of Total Saponins of Panax Notoginseng in Compound Danshen Dropping Pill by High Performance Liquid Chromatography

作　　者：徐美华[1] 李慧珍[1] 朱美晓[1] 吴婷[1] 周军挺[1] 王丽[1] 杨婷婷[1] 鄢连和[1] 

机构地区：[1]丽水市人民医院温州医科大学附属第六医院,浙江丽水323000

出　　处：《中华中医药学刊》2016年第1期221-224,共4页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的：探索效果良好的HPLC条件对中药制剂复方丹参方滴丸中臣药成分三七总皂苷的含量进行检测。方法：色谱条件选择为：流动相的A、B相分别选择乙腈以及蒸馏水,其主要的流动相洗脱过程依次为（0-12-16-25min,80%B-61%B-54%B-80%B,其流速设定为0.6 m L/min,将柱子的温度保持在28℃;分别进行色谱条件确定,对照样品、复方丹参方滴丸以及阴性对照样品色谱图确定以及重复性、稳定性试验,选择一定的进行加样回收率实验。结果：使用HPLC方法对复方丹参方滴丸中三七总皂苷的定量检测实验的稳定性以及重复性均可以满足要求。当三七皂苷R1以及人参皂苷Rg1与Rb1的含量范围依次处于0.023 6～0.512μg、0.042 6～0.89μg、0.035 6～0.716μg时,这时候HPLC分析中样品使用量的高低与对应的峰面积值的大小呈现明显的的线性相关关系。结论：所使用的HPLC方法在复方丹参方滴丸中含有的三七总皂苷的质量分数的检测具有成分分离率较高、峰型明显、杂峰适量少、检测的精准度优等优势,可以在重要药材以及制剂中的有效成分检测中进行广泛的推广使用。Objective： To explore the determination of total saponins of panax notoginseng in Compound Danshen Dropping Pill by HPLC and the test the repeat- ability,stability,etc. Methods： Chromatographic conditions： mobile phase of acetonitrile（ A） and distilled water respectively（ B）,in the main process of gradient elution order： 0 min- 12 min- 16min- 25 min,80% B- 61% B- 54% B- 80% B. Set its velocity at 0. 6 m L / min. Keep the temperature of pillars in 28℃. In Compound Danshen Dropping Pill： total saponins of panax notoginseng detection was as the experimental object,separately determine chromatographic conditions,the control sample,Compound Danshen Dropping Pill and the negative control sample chromatograms and repeat- ability,stability test. Choose some sample recovery rate experiments. Results：The stability and repeat- ability of total saponins of panax notoginseng met the requirements. When the amounts of panax notoginseng saponins R1,Rg1 and Rb1 were 0. 023 6 ～ 0. 512 μg,0. 042 6 ～ 0. 89 μg and 0. 035 6 ～ 0. 716 μg,the discretion of the content of the sample and the corresponding peak area had a good linear relationship. Conclusion： In this paper,the high performance liquid chromatography（ HPLC） has a high separation efficiency on the content determination of total saponins of panax notoginseng in Compound Danshen Dropping Pill. The peak type has the obvious advantage,less impurity peak and high accuracy and can be measured in important medicinal materials and the preparation of active ingredients to wide- ranging use.

关 键 词：复方丹参方滴丸 三七总皂苷 高效液相色谱 

分 类 号：R258.8[医药卫生—中医内科学]