老年患者中血管紧张素原基因多态性与缺血性脑血管疾病的相关性研究  

Correlation of angiotensinogen gene polymorphism with ischemic cerebrovascular disease in elderly patients

作　　者：凌丹芸[1] 曹国良[1] 李晓丽[1] 王传慧[1] 乔红梅[1] 施惠华[1] 冯月英[1] 戴蓉芳[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院老年科,上海201999

出　　处：《世界临床药物》2016年第1期16-20,共5页World Clinical Drug

基　　金：上海交通大学医学院附属第三人民医院院级科研基金资助课题(编号:syz2013-007)

摘　　要：目的 探讨血管紧张素原（AGT）基因多态性与老年患者缺血性脑血管疾病（ICVD）之间的关系。方法 采用基于高温连接酶反应（LDR）的PCR-LDR基因多态性检测方法对50例老年ICVD患者和50例老年健康志愿者进行了AGT基因常见的5个多态性位点检测,分析其相关性、结果 病例组AGT基因SNP位点rs699CC、rs4762CC和rs3789678CC的基因型频率（分别为0.80、0.92和1.00）与对照组的基因型频率（分别为0.58、0.60和0.68）比较,组间差异具有统计学意义（分别为χ~2=6.41,P=0.041;χ~2=15.41,P〈0.001;χ~2=19.048,P〈0.001）;病例组AGT基因SNP位点rs699C、rs4762C和rs3789678C的等位基因频率（分别为0.86、0.94和1.00）和对照组的等位基因频率（分别为0.74、0.77和0.84）比较同样差异具有显著性（分别为χ~2=4.50,P=0.034;χ~2=11.66,P=0.001;χ~2=17.39,P〈0.001）、对其进行风险分析,未见携带其中任一基因型的人发生ICVD的危险性明显增加、结论 AGT基因SNP位点rs699CC、rs4762CC和rs3789678CC基因型可能是老年患者ICVD的独立危险因素。Objective To investigate the association between angiotensinogen （AGT） gene polymorphism and ischemic cerebrovascular disease （ICVD） in elderly patients. Methods AGT gene polymorphism in 50 elderly ICVD patients and 50 healthy people as control was examined through polymerase chain reaction （PCR） amplification and ligase detection reaction （LDR）. Results The MAF genotype frequencies of AGT SNP rs699CC, rs4762CC and rs3 789678CC were 0.80, 0.92 and 1.00 respectively in ICVD group. Compared with those in control group （0.58, 0.60 and 0.68 respectively）, the difference was statistically significant （Z2 = 6.41, P=0.041; Z2 = 15.41, P〈0.001; Z2 = 19.05, P〈0.001）. The allele gene frequencies of rs699C, rs4762C and rs3789678C （0.86, 0.94 and 1.00 respectively） were significantly higher than those （0.74, 0.77 and 0.84） in control （3（2 = 4.50, P =0.034; X2= 11 .66, P=0.001; X2 = 17.39, P〈0.001）. Relative risk analysis showed that the ICVD risk was not related with any genotype. Conclusion The rs699CC, rs4762CC and rs3789678CC genotype ofAGT gene might be an independent risk factor of ICVD in elderly patients.

关 键 词：SNP 血管紧张素原 缺血性脑血管疾病(ICVD) PCR-LDR 

分 类 号：R743[医药卫生—神经病学与精神病学] R394.33[医药卫生—临床医学]