高效液相色谱-串联质谱法测定益母草颗粒中盐酸水苏碱的含量  

Content of stachydrine hydrochloride from Motherwort granule determined by high performance liquid chromatography-tandem mass spectrometry

作　　者：李铭 

机构地区：[1]广西壮族自治区北海食品药品检验所,广西北海536000

出　　处：《中国当代医药》2016年第2期8-10,共3页China Modern Medicine

摘　　要：目的建立高效液相色谱-串联质谱法测定益母草颗粒中盐酸水苏碱含量的方法 。方法 色谱条件采用Agilent SB-C18柱（50 mm×2.1 mm,1.8μm）,以乙腈-0.2%冰乙酸（70︰30）为流动相,流速为0.3 ml/min。质谱检测采用正离子电喷雾离子源（ESI＋）,在多反应监测模式（MRM）下检测,监测离子（m/z）分别为：144/58、144/84。结果盐酸水苏碱浓度在0.2-10.0μg/ml范围内与峰面积呈良好的线性关系（r=0.9992）,平均回收率为102.6%,RSD为1.5%（n=6）。结论 该方法快速简便、专属性强、结果准确,可用于益母草颗粒的质量控制。Objective To establish a method of high performance liquid chromatography（HPLC）-tandem mass spectrometry determining the content of stachydrine hydrochloride from Motherwort granule. Methods The chromatographic conditions were Agilent SB-C18column（50 mm×2.1 mm,1.8 μm）,acetonitrile to 0.2% glacial acetic acid（70︰30） as the mobile phase,and a flow rate of 0.3 ml/min.Positive ion electrospray ionization（ESI＋） was applied for mass spectrometric detection under multiple reaction monitoring（MRM） mode.The monitored ions（m/z） was 144/58,144/84 respectively. Results The concentration of stachydrine hydrochloride being within 0.2 μg/ml to 10.0 μg/ml had a good linear relationship with the peak area.The r value was 0.9992,and the average recovery was 102.6% and the value of RSD was1.5%（n=6）. Conclusion The method is fast,simple,specific,and accurate in outcome,which can be used for quality control of Motherwort granule.

关 键 词：益母草颗粒 盐酸水苏碱 高效液相色谱-串联质谱法 

分 类 号：R284.1[医药卫生—中药学]