铜绿假单胞菌BRC-CXG5菌株的分离鉴定及其对松墨天牛(Monochamus alternatus Hope)幼虫的杀虫活性  

Characterization and Identification of Pseudomonas aeruginosa Strain BRC-CXG5 and its Toxicity to Monochamus alternatus Hope Larva

作　　者：牟亚妮[1] 徐卓吟 翁小倩[2] 黄志成[2] 任文琳[2] 吴松青[3] 

机构地区：[1]福建农林大学生命科学学院,福州350002 [2]福建农林大学生物农药与化学生物学教育部重点实验室,福州350002 [3]福建农林大学林学院,福州350002

出　　处：《生物技术进展》2016年第1期41-46,共6页Current Biotechnology

基　　金：国家林业公益性行业科研专项(201304401);福建农林大学重点项目建设专项(6112C035003);国家大学生创新创业计划项目(201510389025;201510389028);福建省大学生创新创业训练计划项目(201510389101)资助

摘　　要：从松墨天牛(Monochamus alternatus Hope)虫尸中分离获得一株对其有毒力作用的菌株BRC-CXG5,经培养特征观察、形态学鉴定、生理生化指标测定、16S rDNA碱基序列测定和同源性分析、药敏分析以及生物测定,鉴定该菌株属于铜绿假单胞菌属(Pseudomonas aeruginosa)。研究发现,该菌对一些常见药物具有不同程度的敏感性,对松墨天牛幼虫具有杀虫活性。研究结果可为开发新的微生物农药提供材料和理论基础。Pseudomonas aeruginosa strain BRC-CXG5,with cytotoxic activity to Monochamus alternatus Hope larva,was isolated from dead larva. The strain was identified by morphological observation,biochemical and physiological characterization and16 S rDNA sequence analysis,results indicated that BRC-CXG5 was affiliated to Pseudomonas sp.. The strain BRC-CXG5 was identified as Pseudomonas aeruginosa,combined with the 16 S rDNA sequence analysis. BRC-CXG5 showed high cytotoxic activity to Monochamus alternatus Hope larva based on bioassay data. The results provided a material and theoretical base for the effective development of biological pest control agents.

关 键 词：铜绿假单胞菌 杀虫活性 分离鉴定 松墨天牛 

分 类 号：S767.3[农业科学—森林保护学]