芽孢杆菌BRC-XYT1菌株的分离和鉴定及其对松墨天牛(Monochamus alternatus Hope)的杀虫活性  

Isolation and Identification of Bacillus sp. Strain BRC-XYT1 and its Toxicity to Monochamus alternatus Hope

作　　者：熊悦婷 夏枫[2] 黄志成[2] 刘丽琴[2] 翁小倩[2] 吴松青[3] 

机构地区：[1]福建农林大学植物保护学院,福州350002 [2]福建农林大学生物农药与化学生物学教育部重点实验室,福州350002 [3]福建农林大学林学院,福州350002

出　　处：《生物技术进展》2016年第1期47-51,共5页Current Biotechnology

基　　金：国家林业公益性行业科研专项(201304401);福建农林大学重点项目建设专项(6112C035003);国家大学生创新创业计划项目(201510389013;201510389018;201510389023)资助

摘　　要：从枯死木中分离出一株对松墨天牛(Monochamus alternatus Hope)幼虫有毒力作用的菌株BRC-XYT1,基于形态学鉴定、生理生化反应、16S rDNA系统发育分析,鉴定该菌株为芽孢杆菌属(Bacillus)的一个菌种。经药敏分析测试显示,其对于一些常见药物有着不同程度的敏感性。此外,基于生物测定数据,该菌株对松墨天牛幼虫表现出显著的杀虫活性。研究结果为控制松材线虫病(Bursaphelenchus xylophilus)储备了新资源,为开拓新型微生物农药提供了理论基础。In this study,strain BRC-XYT1 with cytotoxic activity to Monochamus alternatus Hope larva,was isolated from withered woods. Based on the morphological observation,biochemical and physiological characterization and phylogenetic analysis of its 16S rDNA gene sequence,BRC-XYT1 was identied as one of the strains of Bacillus sp.. When tested with drug sensitive test,strain BRC-XYT1 possessed different levels of sensitivity to a number of common medicine. Besides,strain BRC-XYT1 showed signicant insecticidal activity to Monochamus alternatus Hope larva based on the bioassay data. This study reserved new resource for controlling pine wilt disease and it also provided a theoretical base for the effective development of novel biological pest control agents.

关 键 词：芽孢杆菌 分离鉴定 松墨天牛 杀虫活性 

分 类 号：S763.306.4[农业科学—森林保护学]