杨树修复阿特拉津污染土壤的根际微生物碳源利用研究  

Microbial Community Characteristics in the Rhizosphere of Atrazine-Contaminated Soil by Dendroremediation of Poplar

作　　者：姚斌[1] 张静茹[1,2] 韦秀文[3] 刘方[2] 陈灿灿[4] 

机构地区：[1]中国林业科学研究院荒漠化研究所,北京100091 [2]贵州大学环境与资源工程学院,贵州贵阳550002 [3]海南省环境科学研究院,海南海口570206 [4]河南理工大学万方科技学院,河南郑州451400

出　　处：《西南林业大学学报（自然科学）》2016年第1期33-37,共5页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然科学基金项目(30700637)资助;中央公益型科研院所基本科研业务费专项资金项目(CAFRIF200706)资助

摘　　要：以杂交杨树作为修复研究对象,利用根箱和Biolog系统开展阿特拉津污染土壤不同酸碱调节剂处理树木修复过程中不同根际微生物多样性研究。结果表明:树木修复初期(第30 d)酸碱调节剂对土壤微生物活性及其群落碳源利用表现出显著的促进作用;而至修复第90 d时,酸性调节剂处理土壤的微生物活性及其群落碳源利用能力开始回落下降,甚至低于单纯植入杨树处理,而碱性调节剂的施用仍然对土壤微生物活性及其微生物群落碳源利用能力具有显著的促进效果。但杨树树木修复阿特拉津污染土壤过程中根际微生物整体活性及其群落结构演变并没有表现出稳定的规律性。鉴于Biolog ECO碳源有限,并不能完全反映土壤微生物群落结构的功能多样性,不同酸碱调节剂处理杨树修复土壤阿特拉津污染过程中根际微生物活性及群落结构不规律变化的原因及相关机理,还有待结合更精密的分析测试手段作进一步深入研究。The change of microbial community characteristics in the different rhizosphere zero of hybrid poplars （Populus deltoids x nigra,DN34） under atrazine stress were investigated by rhizo-box and Biolog system in a green house experiment,with sublimed sulfur and calcium hydroxide as soil pH regulators. Data from measure by Biolog ECO plate demonstrated that the value of average well color development （AWCD） and the carbon source utilization of microorgan- ism in soil amending soil pH regulators was promoter than CK during the initial 30 days; While the results from the 90 days indicated that the activity and the carbon source utilization of soil microbe in acid regulator treatment began to fall down, even lower than that of poplar implant treatment, then the alkaline treatment still stimulated and promoted the ac- tivity and the carbon source utilization ability of soil microbe. Overall, the activity and community structure of soil mi- crobe in rhizosphere regional of atrazine-contaminated soil did not show steady regularity during the phytoremediation process by using hybrid poplars（Populus deltoids x nigra,DN34）. For the reason that the Biolog ECO plate, which only had 31 kinds of carbon sources, could not really indicate the detail information about the function diversity of communitystructure of soil microbe. So it＇s essential for us to take more sophisticated analysis tools for further study.

关 键 词：杨树 根箱 阿特拉津 微生物 平均吸光值 多样性 

分 类 号：S718.8[农业科学—林学]