S对As胁迫下桐花树幼苗巯基化合物含量的影响  

Influence of Sulfur Supply on Thiols in Aegiceras corniculatum(L.)Blanco Under As Stress

作　　者：吴桂容[1,2] 洪华龙 严重玲 

机构地区：[1]滨海湿地生态系统教育部重点实验室(厦门大学),福建厦门361102 [2]贺州学院化学与生物工程学院,广西贺州542800

出　　处：《厦门大学学报（自然科学版）》2016年第1期55-59,共5页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金重点项目(31530008);国家自然科学基金(31370516;31170471)

摘　　要：本研究以红树植物桐花树(Aegiceras corniculatum(L.)Blanco)为实验材料,通过栽培试验研究硫(S)对砷(As)胁迫下桐花树幼苗巯基化合物含量的影响.As含量设为-As(0mg/kg)和+As(60mg/kg)2个处理,而S含量设为-S(0g/kg)、+S(1g/kg)和+HS(2g/kg)3个处理.结果表明:在-As处理组中,桐花树幼苗根、叶中非蛋白巯基(NPT)、谷胱甘肽(GSH)和植物络合素(PCs)等巯基化合物的含量均随着S的施入而有显著升高(p<0.05);+As处理组桐花树幼苗根、叶中NPT和PCs含量在-S、+S、+HS处理下均要显著高于-As处理组(p<0.05);在+As处理组中,桐花树幼苗根、叶中的NPT和叶中PCs含量随着S的施入显著升高(p<0.05),且根中PCs含量与As含量呈显著正相关(r=0.82,p<0.05).上述结果说明As的胁迫和S的施入均能促进巯基化合物的合成,显著提高As胁迫下的桐花树幼苗根、叶中巯基化合物特别是PCs的含量.S在红树植物As抗性中具有重要作用,在As污染下的红树林湿地中施入S可以通过巯基化合物代谢提高红树植物对As的抗性.The influence of sulfur （S） supply on arsenic （As） tolerance and its accumulation in mangrove （Aegiceras corniculatum （L.） Blanco） were evaluated through pot experiments, which were carried out under no As （-As, 0 mg/kg） or under As （.k As, 60 mg/kg） and varied with three levels of S-S （0 g/kg）,＋S （1 g/kg） and＇kHS （2 g/kg）.Results showed that in --As treatments the level of thiols （NPT,GSH and PCs） in roots and leaves of A. corniculatum seedlings increased significantly with S supply （p〈 0.05） ,while the levels of NPT and PCs in roots and leaves of A. corniculatum seedlings in.kAs treatments increased more signifi- cantly than those in -As treatments （p〈0.05）.S supply in＇k As treatments increased the level of NPT and PCs in roots and the level of PCs in leaves of A. corniculatum seedlings （p〈O. 05）.Besides,the level of PCs in roots was positively related with the level of As in roots （r=0.82 ,p〈0. 05）.The results showed that, As stress and S supply could induced the synthesis of thiols,and S sup- ply to A. corniculatum seedlings under As stress could enhanced the synthesis of thiols （especially PCs）.In conclusion, S play an im- portant role in As resistance for mangrove plants,and the supply of S to mangrove wetlands would enhance the resistance of man- grove to As through thiols metabolism.

关 键 词：硫 砷 桐花树 巯基化合物 

分 类 号：Q494[生物学—生理学]