便携扫描式荧光仪的研发  

Developing a Scanning-fluorescence Reader

作　　者：赵忠龙[1] 杨志伟[1] 王海[1] 张晶[2] 闵小平[1,2] 葛胜祥[2] 

机构地区：[1]厦门大学信息科学与技术学院,福建厦门361005 [2]厦门大学国家传染病疫苗与诊断试剂研究工程中心,福建厦门361102

出　　处：《厦门大学学报（自然科学版）》2016年第1期121-125,共5页Journal of Xiamen University：Natural Science

摘　　要：为了能够实现对血液中特定抗原浓度的即时检测(POCT),研制了基于时间分辨荧光免疫分析技术的便携式荧光仪.荧光仪以波长为365nm的紫外LED作为激发光源,经光学结构产生线光斑进行扫描以及激发荧光信号,最后对光电二极管收集的光信号数据进行平滑、求斜率处理.使用该仪器对我们研发的纸条进行检测,并分析其灵敏度,结果表明稀释倍数和检测线面积/控制线面积(AT/AC)之间乘幂关系较好,相关系数为0.994 3.仪器灵敏度、稳定性好,同时成本很低.We developed portable scanning-fluorescence readers based on the time-resolved fluoroimmunoassay in order to quickly measure the concentration of antigen in blood point-of-care testing （POCT）.An ultraviolet LED with laser operating at 365 nm is used in our experiment as the thermal source.The special optical structure can form a strip shot, which generated signal from fluores- cence excitation.The device subsequently addresses waveform data using a gradient smoothing method, We measured the strips that are made by our laboratory, and show that the dilution ratio and AT/AC reach a correlation index of 0. 994 3.The instruments enjoys advantages of high sensitivity, high stability, and low cost.

关 键 词：即时检测 时间分辨荧光免疫分析 扫描式荧光仪 

分 类 号：TH776[机械工程—仪器科学与技术]