正电子发射断层显像与质谱成像互补结合寻找肿瘤相关分子标志物初探  

A pilot study of combining positron emission tomography with mass spectrometry imaging to find molecular biomarkers

作　　者：吴培琳 贺玖明[2] 李铁刚[2] 党永红[1] 再帕尔.阿不力孜 朱朝晖[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院核医学科,北京100730 [2]中国医学科学院北京协和医学院药物研究所天然药物活性物质与功能国家重点实验室,北京100050

出　　处：《现代仪器与医疗》2016年第1期1-4,9,共5页Modern Instruments & Medical Treatment

基　　金：国家重大科学仪器设备开发专项(2011YQ17006710)

摘　　要：正电子发射断层(PET)显像可以活体、无创反映疾病的特征分子改变,如利用^(18)氟-脱氧葡萄糖(^(18)F-FDG)反映肿瘤的葡萄糖代谢,以及利用^(18)氟-硝基咪唑(^(18)F-FMISO)反映肿瘤的乏氧状态等。质谱成像(MSI)不需要标记和复杂准备,可以离体检测到成百上千种分子在病灶内的精确分布信息,但这些分子的功能往往不易明确。本研究利用BALB/c裸鼠4T1乳腺癌肿瘤模型,将PET显像和电喷雾MSI结合,通过图像对比和统计软件分析,分别找到了与PET糖代谢图像和乏氧图像匹配的系列分子,通过查找人类代谢组数据库,初步确定了这些分子的组成。基于以上,本研究初步证实了PET显像和质谱成像互补结合寻找特征分子标志物的可行性。Positron emission tomography （PET） can noninvasively demonstrate the glucose metabolism using 18F-FDG and visualize the hypoxia status in a tumor using 18F-MISO. Mass Spectrometry Imaging （MSI） is a powerful tool to display the distribution of thousands of molecules in situ within a tissue section, with minimal sample preparation and free of labeling. However, it is not easy to specify the related function of each molecule. In this study, we combined PET and Electron Spray Ionization MSI to identify the molecules related to glucose metabolism and hypoxia, using nude mice bearing breast tumor xenograft. The ESI MSI images were treated with dedicated software by referring the microPET images, and serial most related molecules to the 18F-FDG and 18F-FMISO images were sorted out. These molecules were preliminarily identified through the analysis of the Human Metabolome Database. In conclusion, a preliminary method is established to find out the molecules related to a specific molecular image pattern by combining analysis of PET and ESI MSI images. This method holds a promise to seek new candidates as biomarkers of tumor and other diseases, as well.

关 键 词：正电子发射断层 质谱成像 18氟-脱氧葡萄糖 18氟-硝基咪唑 分子标志物 

分 类 号：R445.6[医药卫生—影像医学与核医学]